Following statements describe the characteristics of the enzyme Restriction Endonuclease. Identify the incorrect statement:

1.	The enzyme recognizes a specific palindromic nucleotide sequence in the DNA
2.	The enzyme cuts DNA molecules at an identified position within the DNA
3.	The enzyme binds DNA at specific sites and cuts only one of the two strands
4.	The enzyme cuts the sugar-phosphate backbone at specific sites on each strand

DNA precipitation, out of a mixture of biomolecules, can be achieved by treatment with:
1. Chilled chloroform
2. Isopropanol 
3. Chilled ethanol
4. Methanol at room temperature

Restriction endonuclease:

1. cuts the DNA molecule randomly

2. cuts the DNA molecule at specific sites

3. restricts the synthesis of DNA inside the nucleus

4. synthesis DNA

Restriction endonucleases are enzymes which:

For transformation, microparticles coated with DNA to be bombarded with gene gun are made up of:

1. Silver or platinum

2. Platinum or zinc

3. Silicon or platinum

4. Gold or tungsten

Which one is a true statement regarding DNA polymerase used in PCR?

1. It is used to ligate introduces DNA in recipient cells

2. It serves as a selectable marker

3. It is isolated from a virus

4. It remains active at high temperatures

Which one of the following is a case of wrong matching?

DNA fragments generated by the restriction endonucleases in a chemical reaction can be separated by:

1. Polymerase chain reaction

2. Electrophoresis

3. Restriction mapping

4. Centrifugation

Which of the following is not correctly matched for the organism and its cell wall degrading enzyme?

1. Plant cells- cellulose

2. Algae - Methylase

3. Fungi - Chitinase

4. Bacteria - Lysozyme

The introduction of t-DNA into plants involves?

1. Infection of the plant by Agrobacterium tumefaciens

2. Altering the pH of the soil, heat-shocking the plants

3. Exposing the plants to Cold for a brief period

4. Allowing the plant roots to stand in water