A foreign DNA and plasmid cut by the same restriction endonuclease can be joined to form a recombinant plasmid using?

1.	Eco Rl	2.	taq polymerase
3.	polymerase lll	4.	ligase

Which of the following is not a component of downstream processing?

DNA fragments are:

1. Negatively charged

2. Neutral

3. Either positively charged depending on their size

4. Positively charged

The introduction of t-DNA into plants involves?

1. Infection of the plant by Agrobacterium tumefaciens

2. Altering the pH of the soil, heat-shocking the plants

3. Exposing the plants to Cold for a brief period

4. Allowing the plant roots to stand in water

Which of the following is not correctly matched for the organism and its cell wall degrading enzyme?

1. Plant cells- cellulose

2. Algae - Methylase

3. Fungi - Chitinase

4. Bacteria - Lysozyme

DNA fragments generated by the restriction endonucleases in a chemical reaction can be separated by:

1. Polymerase chain reaction

2. Electrophoresis

3. Restriction mapping

4. Centrifugation

Which one of the following is a case of wrong matching?

Which one is a true statement regarding DNA polymerase used in PCR?

1. It is used to ligate introduces DNA in recipient cells

2. It serves as a selectable marker

3. It is isolated from a virus

4. It remains active at high temperatures