Biolistics

1.	Plant cells are bombarded with high velocity micro-particles of gold or tungsten coated with DNA
2.	Also known as gene gun
3.	Both A and B
4.	Direct injection

pBR322 means

1.      BR for the initials of two scientists who constructed it

2.      BR for the resistance of BamH1

3.      BR for the multiple cloning site

4.     BR  for its discovery in Berlin

The antibiotic resistance gene in pBR322 is not

1.      Tetracycline

2.      Chloramphenicol

3.      Ampicillin

4.     Both A and B

rop segment codes for pBR322?

1.      Resistance protein

2.      Protein for replication

3.      Proteins for translation

4.     Cloning site

Pvu II restriction site is present in pBR322 at?

1.      rop

2.      Tetracycline resistance gene

3.      Ampicillin resistance gene

4.     Ori

BamHI restriction site is present in pBR322 at?

1.      rop

2.      Tetracycline resistance gene

3.      Ampicillin resistance gene

4.     Ori

Sal I restriction site is present in pBR322 at?

1.      rop

2.      Tetracycline resistance gene

3.      Ampicillin resistance gene

4.     Ori

Pvu I restriction site is present in pBR322 at?

1.      rop

2.      Tetracycline Resistance gene

3.      Ampicillin Resistance gene

4.     Ori

If you have a fungal cell and want to extract DNA, what will be the first enzyme to be used?

1.      Chitinase

2.      Lysozyme

3.      Cellulase

4.     Protease

If a researcher starts his work of DNA extraction from a plant cell, the very first enzyme, he would use will be

1.      Cellulase

2.      Protease

3.      Ribonuclease

4.     Chitinase