Pvu I restriction site is present in pBR322 at?

1.      rop

2.      Tetracycline Resistance gene

3.      Ampicillin Resistance gene

4.     Ori

If you have a fungal cell and want to extract DNA, what will be the first enzyme to be used?

1.      Chitinase

2.      Lysozyme

3.      Cellulase

4.     Protease

If a researcher starts his work of DNA extraction from a plant cell, the very first enzyme, he would use will be

1.      Cellulase

2.      Protease

3.      Ribonuclease

4.     Chitinase

If a researcher has to use a bacterial cell to work with after cell wall breaking which enzyme he needs to use?

1.      Cellulase

2.      Chitinase

3.      Lysozyme

4.     Protease

If a researcher has to use a bacterial cell to isolate DNA from bacterial cells then to remove another nucleic acid, which enzyme should be used?

1.      DNAse

2.      Ribonuclease

3.      Protease

4.      Lysozyme

The precipitation of DNA out of the solution is done by adding

1.      Organic Acid

2.      HCl

3.      Ethanol

4.     Ketone

The end result of precipitation of DNA in suspension is seen

1.      In the form of dark solution

2.      In the form of effervescence

3.      In the form of fine threads

4.      In the form of bubbles

The precipitated DNA can be removed from the suspension

1.      By Filtration

2.      By giving a heat shock

3.      By spooling

4.      By gel electrophoresis

Primers are not

1.      Small

2.      Biologically synthesized

3.      Oligonucleotides

4.     Complementary to the regions of DNA

Which of the following doesn't go with PCR?

1.    Thermus aquaticus

2.     1 billion copies

3.     Taq polymerase

4.     Dideoxynucleotides