Primers are not
1. Small
2. Biologically synthesized
3. Oligonucleotides
4. Complementary to the regions of DNA
Which of the following doesn't go with PCR?
1. Thermus aquaticus
2. 1 billion copies
3. Taq polymerase
4. Dideoxynucleotides
If a strand has the following sequence 5’ATTGCCCTAG 3’what will be the sequence of DNA synthesized from its complementary strand.
1. 5’TAACGGGATC 3’
2. 3’TAACGGGATC 5’
3. 5’ATTGCCCTAG 3’
4. 3’ATTGCCCTAG 5’
If you start with 10 molecules of Double stranded DNA, what will be the number of DNA molecules formed after 10 cycles of PCR?
1. 10240
2. 1024
3. 512
4. 5120
What is the final step of PCR?
1. Extension
2. Annealing
3. Denaturation
4. Extraction
After 30 cycles of PCR, how many times DNA will be amplified?
1. Thousand times
2. Million times
3. Billion times
4. Hundreds times
Use of primers, occurs in which step of PCR?
1. Annealing
2. Extension
3. Denaturation
4. Both extension and Annealing
The ultimate aim of maximum recombinant DNA technologies is
1. To multiply DNA
2. To express DNA
3. To multiply mRNA
4. To express protein
Which of the following processes is not associated with the protein produced by recombinant DNA?
1. Restriction Digestion
2. Expression
3. Optimization
4. Purification
What does continuous culture mean?
1. Where DNA and protein getting expressed continuously
2. Where used medium is drained out and the fresh medium is added from another side
3. Where cells are producing protein for one week continuously
4. Where the production of recombinant DNA is continuing without interference.