Micro-injection is a method of introducing alien DNA into
1. Cytoplasm of all cells
2. Cytoplasm of bacterial cell
3. Nucleus of animal cell
4. Nucleus of plant cell
Biolistics
1. | Plant cells are bombarded with high velocity micro-particles of gold or tungsten coated with DNA |
2. | Also known as gene gun |
3. | Both A and B |
4. | Direct injection |
pBR322 means
1. BR for the initials of two scientists who constructed it
2. BR for the resistance of BamH1
3. BR for the multiple cloning site
4. BR for its discovery in Berlin
The antibiotic resistance gene in pBR322 is not
1. Tetracycline
2. Chloramphenicol
3. Ampicillin
4. Both A and B
rop segment codes for pBR322?
1. Resistance protein
2. Protein for replication
3. Proteins for translation
4. Cloning site
Pvu II restriction site is present in pBR322 at?
1. rop
2. Tetracycline resistance gene
3. Ampicillin resistance gene
4. Ori
BamHI restriction site is present in pBR322 at?
1. rop
2. Tetracycline resistance gene
3. Ampicillin resistance gene
4. Ori
Sal I restriction site is present in pBR322 at?
1. rop
2. Tetracycline resistance gene
3. Ampicillin resistance gene
4. Ori
Pvu I restriction site is present in pBR322 at?
1. rop
2. Tetracycline Resistance gene
3. Ampicillin Resistance gene
4. Ori
If you have a fungal cell and want to extract DNA, what will be the first enzyme to be used?
1. Chitinase
2. Lysozyme
3. Cellulase
4. Protease