EcoRI cuts

1.      A little away from the centre

2.      Both the strands at the same time

3.      Creates blunt ends

4.      Both 1 and 2

DNA gel electrophoresis is commonly prepared by

1.      Agarose, a natural polymer

2.      Extracted from seaweeds

3.      A synthetic polymer

4.      Both 1 and 2

Which of the following step is first one in order to separate DNA fragments?

1.      Running of gel

2.      Staining of gel

3.      Elution

4.     Setting of gel

Which of the following step is last for isolating the desired DNA fragment?

1.      Running of gel

2.      Elution

3.      Staining

4.     Visualization

Arrange the following steps in order:

1) Restriction Digestion
2) Running Agarose gel
3) Elution of bands
4) Purification of DNA
5) ligation
6) transformation
7) Cloning

1.      1-2-3-4-5-6-7

2.      1-5-6-7-2-3-4

3.      1-2-3-5-6-7-4

4.      2-3-4-1-5-6-7

Which of the following steps will be performed after restriction digestion?

1.      Ligation

2.      Elution from DNA gel

3.      Transformation

4.      Cloning

If we are able to link an alien piece of DNA with ……a…...We can multiply it's number equal to the copy number of

…a…..

1. a- bacteriophage

2. a- Plasmid

3. a- bacteriophage or Plasmid

4. a- prion

Which of the following is not a feature to facilitate cloning into a vector?

1.      Origin of replication

2.      Selectable Marker

3.      Multiple cloning site

4.      Virulence region

If I want to check the expression of my gene, then I should use

1.      Vectors supporting high copy number

2.      Vectors supporting low copy number

3.      No need to bother about copy number

4.     Vectors with strong Ori

Selectable marker helps

1.      To identify transformants

2.      To identify recombinants

3.      To identify non- recombinants

4.      To eliminate transformants