Arrange the following steps in order:

1) Restriction Digestion
2) Running Agarose gel
3) Elution of bands
4) Purification of DNA
5) ligation
6) transformation
7) Cloning

1.      1-2-3-4-5-6-7

2.      1-5-6-7-2-3-4

3.      1-2-3-5-6-7-4

4.      2-3-4-1-5-6-7

Which of the following steps will be performed after restriction digestion?

1.      Ligation

2.      Elution from DNA gel

3.      Transformation

4.      Cloning

If we are able to link an alien piece of DNA with ……a…...We can multiply it's number equal to the copy number of

…a…..

1. a- bacteriophage

2. a- Plasmid

3. a- bacteriophage or Plasmid

4. a- prion

Which of the following is not a feature to facilitate cloning into a vector?

1.      Origin of replication

2.      Selectable Marker

3.      Multiple cloning site

4.      Virulence region

If I want to check the expression of my gene, then I should use

1.      Vectors supporting high copy number

2.      Vectors supporting low copy number

3.      No need to bother about copy number

4.     Vectors with strong Ori

Selectable marker helps

1.      To identify transformants

2.      To identify recombinants

3.      To identify non- recombinants

4.      To eliminate transformants

The normal E. coli cells have

1.      Kanamycin

2.      Tetracycline

3.      Ampicillin

4.     No resistance

For easiest cloning, one needs to get vector with

1.      Single cloning site

2.      Multiple cloning site

3.      Few cloning site

4.     No cloning site at all

If the gene of Beta-galactosidase is not inactivated, means there will be color in the colonies of

1.      Non- recombinant

2.      Recombinant

3.      Transformants

4.     Non-transformants

Insertional inactivation results in

1.      Non-recombinants

2.      Recombinants

3.      Transformants

4.     Non- transformants