The precipitation of DNA out of the solution is done by adding

A.      Organic Acid

B.      HCl

C.      Ethanol

D.     Ketone

The end result of precipitation of DNA in suspension is seen

A.      In the form of dark solution

B.      In the form of effervescence

C.      In the form of fine threads

D.     In the form of bubbles

The precipitated DNA can be removed from the suspension

A.      By Filtration

B.      By giving a heat shock

C.      By spooling

D.     By gel electrophoresis

The synthesis of DNA in PCR will be in

1. 5’-3’

2. 3’-5’

3. It won't be depending on direction

4. For one strand 5’-3’ in another opposite to it

The primers designed according to (Answer according to very basic PCR)

1. Terminal ends of vector

2. Terminal ends of gene of interest

3. Random primers

4. According to direction of PCR

Which of the following doesn't go with PCR?

A.      Thermus aquaticus

B.      1 billion copies

C.      Taq polymerase

D.     Dideoxynucleotides

What is the final step of PCR?

A.      Extension

B.      Annealing

C.      Denaturation

D.     Extraction

After 30 cycle of PCR, how many times DNA will be amplified?

A.      Thousand times

B.      Million times

C.      Billion times

D.     Hundreds times

Use of primers, occurs in which step of PCR?

A.      Annealing

B.      Extension

C.      Denaturation

D.     Both extension and Annealing

If you have transformed cell with recombinant DNA and it grows on kanamycin and chloramphenicol containing plates but does not grow on ampicillin containing medium. What does it imply?

A.      The cell has resistance against ampicillin but not against chloramphenicol and kanamycin

B.      Has resistance against both kanamycin and chloramphenicol but not against ampicillin

C.      We may not confer anything from it

D.     It depends upon gene of interest