The primers designed according to (Answer according to very basic PCR)

1. Terminal ends of vector

2. Terminal ends of gene of interest

3. Random primers

4. According to direction of PCR

Which of the following doesn't go with PCR?

A.      Thermus aquaticus

B.      1 billion copies

C.      Taq polymerase

D.     Dideoxynucleotides

What is the final step of PCR?

A.      Extension

B.      Annealing

C.      Denaturation

D.     Extraction

After 30 cycle of PCR, how many times DNA will be amplified?

A.      Thousand times

B.      Million times

C.      Billion times

D.     Hundreds times

Use of primers, occurs in which step of PCR?

A.      Annealing

B.      Extension

C.      Denaturation

D.     Both extension and Annealing

If you have transformed cell with recombinant DNA and it grows on kanamycin and chloramphenicol containing plates but does not grow on ampicillin containing medium. What does it imply?

A.      The cell has resistance against ampicillin but not against chloramphenicol and kanamycin

B.      Has resistance against both kanamycin and chloramphenicol but not against ampicillin

C.      We may not confer anything from it

D.     It depends upon gene of interest

The ultimate aim of maximum recombinant DNA technologies is

1. To multiply DNA

2. To express DNA

3. To multiply mRNA

4. To express protein

Which of the following processes is not associated with protein produced by recombinant DNA?

A.      Restriction Digestion

B.      Expression

C.      Optimization

D.     Purification

What does continuous culture mean?

A.      Where DNA and protein getting expressed continuously

B.      Where used medium is drained out and fresh medium is  added from other side

C.      Where cells are producing protein for one week continuously

D.     Where production of recombinant DNA is continuing without interference.

Continuous culture leads to

1. Smaller biomass

2. Higher yields of desired proteins

3. Both A and B

4. Higher yields of recombinant DNA