Agrobacterium tumifaciens

1. A pathogen of  angiosperms

2. It is able to deliver a piece of DNA known as T-DNA to transform bacterial cell and direct the cell to produce chemicals required by the pathogen

3. Ti plasmid of it is now modified into an expression vector

4. It's Ti plasmid is modified into a cloning vector which is no more pathogenic

Subtopic:  Transforming Plant & Animal Cell |
 54%
From NCERT
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In order to make the host cell competent

Divalent cations such as calcium are used-

A.      The divalent ions has to be in specific concentration

B.      They cause the DNA uptake by the cell

C.      They increase the efficiency with which DNA enters the bacterium through pores in its cell wall.

D.     Both B and C

Subtopic:  Host & Desired DNA: I | Host & Desired DNA |
 83%
From NCERT
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rop segment codes for pBR322?

1. Resistance protein

2. Protein for replication

3. Proteins for translation

4. Cloning site

Subtopic:  Cloning Vector |
 74%
From NCERT
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If a researcher start his work of DNA extraction from a plant cell, the very first enzyme, he would use will be

A.      Cellulase

B.      Protease

C.      Ribonuclease

D.     Chitinase

Subtopic:  Host & Desired DNA: II | Host & Desired DNA |
 94%
From NCERT
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If a researcher has to use a bacterial cell to work with after cell wall breaking which enzyme he needs to use?

A.      Cellulase

B.      Chitinase

C.      Lysozyme

D.     Protease

Subtopic:  Host & Desired DNA: II | Host & Desired DNA |
 86%
From NCERT
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If a researcher has to use a bacterial cell to isolate DNA from bacterial cell then to remove other nucleic acid, which enzyme should be used?

A.      DNAse

B.      Ribonuclease

C.      Protease

D.     Lysozyme

Subtopic:  Host & Desired DNA: II | Host & Desired DNA |
 62%
From NCERT
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The precipitation of DNA out of the solution is done by adding

A.      Organic Acid

B.      HCl

C.      Ethanol

D.     Ketone

Subtopic:  Host & Desired DNA: II | Host & Desired DNA |
 97%
From NCERT
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The end result of precipitation of DNA in suspension is seen

A.      In the form of dark solution

B.      In the form of effervescence

C.      In the form of fine threads

D.     In the form of bubbles

Subtopic:  Host & Desired DNA: II | Host & Desired DNA |
 88%
From NCERT
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The precipitated DNA can be removed from the suspension

A.      By Filtration

B.      By giving a heat shock

C.      By spooling

D.     By gel electrophoresis

Subtopic:  Host & Desired DNA: II | Host & Desired DNA |
 88%
From NCERT
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The synthesis of DNA in PCR will be in

1. 5’-3’

2. 3’-5’

3. It won't be depending on direction

4. For one strand 5’-3’ in another opposite to it

Subtopic:  Large Scale Production: I | Large Scale Production |
 59%
From NCERT
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