rop segment codes for pBR322?

1.      Resistance protein

2.      Protein for replication

3.      Proteins for translation

4.     Cloning site

Pvu II restriction site is present in pBR322 at?

1.      rop

2.      Tetracycline resistance gene

3.      Ampicillin resistance gene

4.     Ori

BamHI restriction site is present in pBR322 at?

1.      rop

2.      Tetracycline resistance gene

3.      Ampicillin resistance gene

4.     Ori

Sal I restriction site is present in pBR322 at?

1.      rop

2.      Tetracycline resistance gene

3.      Ampicillin resistance gene

4.     Ori

Pvu I restriction site is present in pBR322 at?

1.      rop

2.      Tetracycline Resistance gene

3.      Ampicillin Resistance gene

4.     Ori

If you have a fungal cell and want to extract DNA, what will be the first enzyme to be used?

1.      Chitinase

2.      Lysozyme

3.      Cellulase

4.     Protease

If a researcher starts his work of DNA extraction from a plant cell, the very first enzyme, he would use will be

1.      Cellulase

2.      Protease

3.      Ribonuclease

4.     Chitinase

If a researcher has to use a bacterial cell to work with after cell wall breaking which enzyme he needs to use?

1.      Cellulase

2.      Chitinase

3.      Lysozyme

4.     Protease

If a researcher has to use a bacterial cell to isolate DNA from bacterial cells then to remove another nucleic acid, which enzyme should be used?

1.      DNAse

2.      Ribonuclease

3.      Protease

4.      Lysozyme

The precipitation of DNA out of the solution is done by adding

1.      Organic Acid

2.      HCl

3.      Ethanol

4.     Ketone