Which of the following options is correct about Agrobacterium tumifaciens?

1.     It is a pathogen of angiosperms

2.     It is able to deliver a piece of DNA known as T-DNA to transform bacterial cell and direct the cell to produce chemicals required by the pathogen

3.     Its Ti plasmid is now modified into an expression vector

4.     Its Ti plasmid is modified into a cloning vector which is no more pathogenic

In order to make the host cell competent

Divalent cations such as calcium are used-

1.      The divalent ions have to be in a specific concentration

2.      They cause the DNA uptake by the cell

3.      They increase the efficiency with which DNA enters the bacterium through pores in its cell wall.

4.     All of the above

The process of competent cell formation include

1.      CaCl2 treatment- ice incubation with r-DNA, heat shock at 42 degree Celsius, ice incubation

2.      Ice incubation - CaCl2 treatment - Heat shock-Ice incubation with r-DNA

3.      CaCl2 treatment - Heat shock -Ice incubation with r-DNA

4.     Ice incubation - CaCl2 treatment - Heat treatment at 45 degree Celsius, Ice incubation with r-DNA

Micro-injection is a method of introducing alien DNA into

1.      Cytoplasm of all cells

2.      Cytoplasm of bacterial cell

3.      Nucleus of animal cell

4.     Nucleus of plant cell

Biolistics

pBR322 means

1.      BR for the initials of two scientists who constructed it

2.      BR for the resistance of BamH1

3.      BR for the multiple cloning site

4.     BR  for its discovery in Berlin

The antibiotic resistance gene in pBR322 is not

1.      Tetracycline

2.      Chloramphenicol

3.      Ampicillin

4.     Both A and B

rop segment codes for pBR322?

1.      Resistance protein

2.      Protein for replication

3.      Proteins for translation

4.     Cloning site

Pvu II restriction site is present in pBR322 at?

1.      rop

2.      Tetracycline resistance gene

3.      Ampicillin resistance gene

4.     Ori

BamHI restriction site is present in pBR322 at?

1.      rop

2.      Tetracycline resistance gene

3.      Ampicillin resistance gene

4.     Ori