Which is a natural genetic engineer of the plants?
(1)E.coli
(2)A. tumefaciens
(3)Rhizobium
(4)Pseudomonas
The thermostable enzyme Taq polymerase used in PCR technique was discovered by
(1)Alec Jeffrey
(2)Karry Mullis
(3)Temin
(4)Baltimore
To be useful in genetic engineering a vector must have
In 1960’s two enzymes were discovered in bacteria that were responsible for providing immunity against bacteriophages. One was Restriction Endonuclease and the other was
1. Methylase
2. Exonuclease
3. Aminotransferase
4. Terminal Transferase
E.coli is a commonly used host for gene cloning because
1. It is free from elements that interfere with replication and recombination of DNA
2. It is easy to transform
3. It supports replication of inserted DNA.
4. All of these
A gene has been inserted into a vector for the purpose of cloning the sequence only. This vector is called
a. Suppression vector
b. Expression vector
c. Cloning vector
d. None of these
Electroporation facilitates insertion of foreign DNA into the cell by
1. Changing the porocity of the membrane
2. Changing the electrical potential of the membrane
3. Lysing the cell wall
4. Active transport
The names associated with construction of first Recombinant DNA are
a. Arber, Nathans, Smith
b. Annie Chang, Boyer, Berg, Cohen
c. Howard Temin, Brenner, Sharp
d. Tim Hunt, Hartwell, Nurse
The restriction enzymes most commonly used in rDNA technology are
1. Type 1 Restriction enzymes
2. Type II Restriction enzymes
3. Type III Restriction enzymes
4. Type IV Restriction enzymes
Restriction enzymes do not act on the the DNA of the Host cell in which they are produced because
a. Host DNA is packed into chromosomes
b. Restriction enzymes are ineffective on host DNA
c. Host DNA does not have the restriction site for the Restriction enzymes.
d. Restriction site of host DNA is methylated.