Given below is a sample of portion of DNA strand giving the base sequence on the

opposite strands. What is so, special shown in it?

5'__ GAATTC_ 3'

3' __ CTTAAG__5'

1. Deletion mutation

2. Start codon at the 5' end

3. Palindromic sequence of base pairs

4. Replication completed

There is a restriction endonuclease called Eco RI. What does 'co' part in it stand for?

1. Coelom                         

2. Coenzyme

3. coli                               

4. Colon

Agarose extracted from sea weeds finds use in

1. tissue culture

2. PCR

3. gel electrophoresis

4. spectrophotometry

Continuous addition of sugars in ‘fed batch’ fermentation is done to

1. obtain antibiotics

2. purify enzymes

3. degrade sewage

4. produce methane

Which one of the following is used as vector for cloning genes into higher organisms?

1. Baculovirus

2. Salmonella typhimurium

3. Rhizopus nigricans

4. Retrovirus

Which one of the following palindromic base sequences in DNA can be easily cut at about

the middle by some particular restriction enzyme?

1. 5'$-$CGTTCG$-$3'

     3'$-$ATGGTA$-$-5'

2. 5'$-$GATATG$-$3'

     3'$-$CTACTA$-$5'

3. 5'$-$GAATTC$-$3'

     3'$-$CTTAAG$-$5'

4. 5'$-$CACGTA$-$3'

     3'$-$CTCAGT$-$5'

Restriction endonucleases are enzymes which

1. make cuts at specific positions within the DNA molecule

2. recognize a specific nucleotide sequence for binding of DNA ligase

3. restrict the action of the enzyme DNA polymerase

4. remove nucleotides from the ends of the DNA molecule

Stirred-tank bioreactors have been designed for

1. addition of preservatives of the product

2. purification of the product

3. ensuring anaerobic conditions in the culture vessel

4. availability of oxygen throughout the process

Which one of the following is commonly used in transfer of foreign DNA into crop plants?

1. Trichoderma harzianum

2. Meloidogyne incognita

3. Agrobacterium tumefaciens

4. Penicillium expansum

Polyethylene glycol method is used for

1. gene transfer without a vector

2. biodiesel production

3. seedless fruit production

4. energy production from sewage