Which of the following restriction sites is located within the gene for tetracycline resistance in the plasmid pBR322?
1. BamHI
2. Psti
3. Clal
4. Pvull
The technique not used for transformation of plant cells in recombinant procedures is:
1. Biolistics
2. Agrobacterium mediation
3. Use of viruses
4. Micro-injection
In the screening process during rDNA experiments, clones that metabolize -gal turn:
1. Colorless
2. Blue
3. Yellow
4. Green
Microinjection is suitable for :
1. Injecting an ovum into the sperm in IVF
2. Transforming animal cells
3. Injecting very small sized drug particles into neurons
4. Conferring antibiotic resistance to a certain strain of bacteria
The plasmid pBR322 does not contain:
1. An origin of replication site
2. A gene that encodes for restrictor of plasmid copy number
3. Gene for Ampicillin and Streptomycin resistance
4. Sites for many restriction enzymes
Restriction enzymes are synthesized by:
1. Bacteria only
2. Yeast and bacteria only
3. Eukaryotic cells only
4. All kinds of cells
The bioreactor shown in the diagram is:
1. Sparged stirred tank type
2. Airlift type
3. Simple stirred tank type
4. Fluidized bed type
What is true about the steps of PCR shown in the diagram given below?
l. Step A occurs at the temperature of 95-98 C
ll. Step B occurs at the temperature of 55 C
lll. Step C occurs at the temperature of 72 C
1. l and ll only
2. l and lll only
3. ll and lll only
4. l, ll and lll
A and B in the pBR 322, shown in the diagram given below, respectively represent recognition sequences of:
1. BamH I and Sma I
2. Hind II and Sma I
3. BamH I and SaI I
4. SaI I and Hind II
What is true A,B and C in the given diagrammatic representation of rDNA technology ?
I. At A same restriction enzyme is used to cut both foreign and vector DNA
II. The enzyme used at B is DNA ligase
III. Step C can be called as transformation
1. I and II only
2. I and III only
3. II and III only
4. I,II and III