The enzyme used in the polymerase chain reaction is a :
(1) DNA dependent RNA polymerase
(2) RNA dependent DNA polymerase
(3) DNA dependent DNA polymerase
(4) RNA dependent RNA polymerase
Taq polymerase is used in the polymerase chain reaction because:
1. It replicates DNA faster than other enzymes
2. It is the only enzyme that can replicate DNA invitro
3. It does not require an RNA primer to function
4. It is thermostable
What is true about the steps of PCR shown in the diagram given below?
l. Step A occurs at the temperature of 95-98 C
ll. Step B occurs at the temperature of 55 C
lll. Step C occurs at the temperature of 72 C
1. l and ll only
2. l and lll only
3. ll and lll only
4. l, ll and lll
Which one is a true statement regarding DNA polymerase used in PCR?
1. It is used to ligate introduced DNA in recipient cells
2. It serves as a selectable marker
3. It is isolated from a virus
4. It remains active at high temperature
Select the correct order of processing of PCR.
(1) Extension, primer annealing, denaturation
(2) Denaturation, primer annealing, extension
(3) Denaturation, extension, primer annealing
(4) Primer annealing, denaturation, extension