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Q. No.The European Federation of Biotechnology [EFB] defines biotechnology as:
| 1. | The use of living cells and bacteria in industrial and scientific processes. |
| 2. | The integration of natural sciences and organisms, cells, parts thereof, and molecular analogues for products and services. |
| 3. | The use of biology to solve problems and make useful products. |
| 4. | The use of biology to develop new products, methods and organisms intended to improve human health and society. |
Subtopic: Introduction & History: I |
89%
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Bioprocessing engineering deals with:
| 1. | the search for plant and animal species from which medicinal drugs and other commercially valuable compounds can be obtained. |
| 2. | developing methods and software tools for understanding biological data. |
| 3. | production of active pharmaceutical substances in genetically modified organisms (GMOs). |
| 4. | maintenance of sterile ambience in chemical engineering processes. |
Subtopic: Introduction & History: I |
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What limitation of traditional hybridization procedures used in plant and animal breeding can be overcome by recombinant DNA procedures?
| I: | Inclusion and multiplication of undesirable genes |
| II: | Inability of inclusion of desirable traits from other species |
| 1. | Only I | 2. | Only II |
| 3. | Both I and II | 4. | Neither I nor II |
Subtopic: Selectable Markers | Tools |
68%
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A piece of DNA, somehow transferred into an alien organism, will be able to multiply itself in the progeny cells of organism if:
| 1. | It includes the centromere |
| 2. | It becomes a part of chromosome |
| 3. | It does not contain any intron sequences |
| 4. | It is a single stranded polynucleotide |
Subtopic: Cloning Vector |
86%
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For the multiplication of any alien piece of DNA in an organism, it needs to be part of a chromosome that has a specific:
| 1. | Telomeric sequence | 2. | Multiple cloning sites |
| 3. | Ori | 4. | Selectable marker |
Subtopic: Cloning Vector |
89%
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The work of which of the following scientists led to the establishment of the discipline of modern biotechnology?
| 1. | Banting and Best | 2. | Bolivar and Rodriguez |
| 3. | Hershey and Chase | 4. | Cohen and Boyer |
Subtopic: Restriction Enzymes - Main Enzymes |
84%
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In recombinant DNA experiments, a vector:
| 1. | carries DNA into a new cell |
| 2. | links together newly joined fragments of DN |
| 3. | makes millions of copies of a specific segment of DNA |
| 4. | separates fragments of DNA by their length and electrical charges |
Subtopic: Cloning Vector |
77%
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The palindrome in DNA is a sequence of base pairs:
| 1. | that reads same on the two strands when orientation of reading is kept the same. |
| 2. | that reads same on the two strands when orientation of reading is 5’ to 3’ on one end and 3’ to 5’ on the other. |
| 3. | that are complementary on the two strands when orientation of reading is kept the same. |
| 4. | that are complementary on the two strands when orientation of reading is 5’ to 3’ on one end and 3’ to 5’ on the other. |
Subtopic: Restriction Enzymes - Main Enzymes |
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Identify the incorrect statement regarding restriction endonucleases:
| 1. | They mostly cut dsDNA at specific base sequences. |
| 2. | They are produced by bacterial cells as a mechanism of self-defence. |
| 3. | They digest DNA by removing nucleotides from a free 3' end. |
| 4. | They often generate short single-stranded sequences at the ends of the resultant fragments. |
Subtopic: Restriction Enzymes - Main Enzymes |
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The first type II restriction endonuclease whose functioning depended on a specific DNA nucleotide sequence was:
| 1. | EcoRI | 2. | HindII |
| 3. | SmaI | 4. | BamHI |
Subtopic: Tools: Enzymes: I | Tools: Enzymes: II |
91%
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