Plasmid pUC18 carries lacZ gene that confers the host cells to convert white X-gal into blue indole. A gene is inserted within the lacZ gene of the plasmid. Bacterial cells transformed with this rDNA, if provided with X-gal in the medium, will produce:

1.  Blue colonies
2. White colonies
3. No colonies
4. Orange colonies if seen through UV light
Subtopic:  Selection of Recombinant Transformants |
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