Stirred-tank bioreactors have been designed for:
1. Addition of preservatives to the product
2. Purification of the product
3. Ensuring anaerobic conditions in the culture vessel
4. Availability of oxygen throughout the process
1. | cutting of DNA into fragments |
2. | separation of DNA fragments according to their size |
3. | construction of recombinant DNA by joining with cloning vectors |
4. | isolation of DNA molecule |
1. DNA ligase
2. endonucleases
3. DNA polymerase
4. exonucleases
Restriction endonuclease:
1. cuts the DNA molecule randomly
2. cuts the DNA molecule at specific sites
3. restricts the synthesis of DNA inside the nucleus
4. synthesis DNA
Restriction endonucleases are enzymes which:
1. | make cuts at specific positions within the DNA molecule |
2. | recognize a specific nucleotide sequence for binding of DNA ligase |
3. | restrict the action of the enzyme DNA polymerase |
4. | remove nucleotides from the ends of the DNA molecule |
Which one of the following palindromic base sequences in DNA can be easily cut at about the middle by some particular restriction enzyme?
1. | 5'-CGTTCG-3' | 2. | 5'-GATATG-3' |
3'-ATGGTA-5' | 3'-CTACTA-5' | ||
3. | 5'-GAATTC-3' | 4. | 5'-CACGTA-3' |
3'-CTTAAG-5' | 3'-CTCAGT-5' |
1. obtain antibiotics
2. purify enzymes
3. degrade sewage
4. produce methane
1. Deletion mutation
2. Start codon at the 5' end
3. Palindromic sequence of base pairs
4. Replication completed