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Q. No.Identify the incorrect statement related to electrophoresis:
1.
Separated DNA fragments can be directly seen under UV radiation
2.
Separated DNA can be extracted from gel piece.
3.
Fragments of DNA moves toward anode.
4.
Sieving effect of agarose gel helps in separation of DNA fragments.
Subtopic: Separation and Isolation of DNA fragments |
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Select the restriction endonuclease enzymes whose restriction sites are present for the tetracycline resistance (tetR) gene in the pBR322 cloning vector.
1. Bam HI and Sal I
2. Sal I and Pst I
3. Pst I and Pvu I
4. Pvu I and Bam HI
1. Bam HI and Sal I
2. Sal I and Pst I
3. Pst I and Pvu I
4. Pvu I and Bam HI
Subtopic: Cloning Vector |
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Recombinant DNA molecule can be created normally by cutting the vector DNA and source DNA respectively with:
1. Hind II, Hind II
2. Hind II, Alu I
3. Hind II, EcoR I
4. Hind II, Bam HI
1. Hind II, Hind II
2. Hind II, Alu I
3. Hind II, EcoR I
4. Hind II, Bam HI
Subtopic: Tools: Enzymes: I | Tools: Enzymes: II |
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Which of the following are correct about EcoR1?
Choose the correct answer from the options given below:
1. B,C,E only
2. A,D,E only
3. A,C,D only
4. B,C,D only
| A. | Cut the DNA with blunt end |
| B. | Cut the DNA with sticky end |
| C. | Recognises a specific palindromic sequence. |
| D. | Cut the DNA between the base G and A where encounters the DNA sequence 'GAATTC' |
| E. | Exonuclease |
1. B,C,E only
2. A,D,E only
3. A,C,D only
4. B,C,D only
Subtopic: Restriction Enzymes - Main Enzymes |
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Following are the steps involved in the process of PCR.
A. Annealing
B. Amplification (~1 billion times)
C. Denaturation
D. Treatment with Taq polymerase and deoxynucleotides
E. Extension
Choose the correct sequence of steps of PCR from the options given below :
1. C → A → D → E → B
2. A → B → E → D → C
3. A → C → E → D → B
4. D → B → E → C → A
A. Annealing
B. Amplification (~1 billion times)
C. Denaturation
D. Treatment with Taq polymerase and deoxynucleotides
E. Extension
Choose the correct sequence of steps of PCR from the options given below :
1. C → A → D → E → B
2. A → B → E → D → C
3. A → C → E → D → B
4. D → B → E → C → A
Subtopic: Polymerase Chain Reaction: PCR |
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In a chromosome, there is a specific DNA sequence, responsible for initiating replication. It is called as:
1. recognition sequence
2. cloning site
3. restriction site
4. ori site
1. recognition sequence
2. cloning site
3. restriction site
4. ori site
Subtopic: Principles of Biotechnology |
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Given below are two statements:
In light of the above statements, choose the correct answer from the options given below:
1. Both Statement I and Statement II are True
2. Both Statement I and Statement II are False
3. Statement I is True but Statement II is False
4. Statement I is False but Statement II is True
| Statement I: | Restriction Endonuclease finds its specific recognition sequence and binds to the DNA. |
| Statement II: | Restriction Endonuclease cuts each of the two strands of the double helix at specific points in their sugar phosphate backbones. |
1. Both Statement I and Statement II are True
2. Both Statement I and Statement II are False
3. Statement I is True but Statement II is False
4. Statement I is False but Statement II is True
Subtopic: Restriction Enzymes - Main Enzymes |
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Match List-I with List-II
Choose the correct answer from the options given below:
1. A-III, B-IV, C-I , D-II
2. A-III, B-I, C-IV, D-II
3. A-IV, B-III, C-II , D-I
4. A-II, B-IV, C-I, D-III
| List-I | List-II | ||
| A. | Ampicillin | I. | Restriction sites |
| B. | Ori | II. | Blue/White screening |
| C. | Multiple cloning sites | III. | Selectable marker |
| D. | \(\beta\) galactosidase gene | IV. | Sequence from where replication starts |
Choose the correct answer from the options given below:
1. A-III, B-IV, C-I , D-II
2. A-III, B-I, C-IV, D-II
3. A-IV, B-III, C-II , D-I
4. A-II, B-IV, C-I, D-III
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Which of the following is not a selectable marker of cloning vectors?
1. Ampicillin
2. Metformin
3. Chloramphenicol
4. Tetracycline
1. Ampicillin
2. Metformin
3. Chloramphenicol
4. Tetracycline
Subtopic: Cloning Vector |
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The following diagram showing restriction sites in E.coli cloning vector pBR322. Find the role of 'X' and 'Y' genes :
| 1. | The gene 'X' is responsible for controlling the copy number of the linked DNA and 'Y' for protein involved in the replication of Plasmid. |
| 2. | The gene 'X' is 'for protein involved in replication of Plasmid and 'Y' for resistance to antibiotics. |
| 3. | Gene 'X' is responsible for recognition sites and 'Y' is responsible for antibiotic resistance. |
| 4. | The gene 'X' is responsible for resistance to antibiotics and 'Y' for protein involved in the replication of Plasmid. |
Subtopic: Cloning Vector |
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