Q. No.A gene has been inserted into a vector for the purpose of cloning the sequence only. This vector is called
1.
Suppression vector
2.
Expression vector
3.
Cloning vector
4.
None of these
The names associated with construction of first Recombinant DNA are
| 1. | Arber, Nathans, Smith |
| 2. | Annie Chang, Boyer, Berg, Cohen |
| 3. | Howard Temin, Brenner, Sharp |
| 4. | Tim Hunt, Hartwell, Nurse |
Insertional inactivation of a gene helps in
| 1. | Identification of recombinant clones |
| 2. | Identification of deletion mutants |
| 3. | Identification of recombinant transformants |
| 4. | Elimination of suppression mutants |
What is true about the DNA polymerase used in PCR?
| 1. | Isolated from a virus |
| 2. | Stable at high temperatures |
| 3. | Used as selectable marker |
| 4. | Has methylated bases |
Biolistic method makes use of microparticles coated with DNA bombarded at cells to be transformed. These particle are made up of
| 1. | Zinc or tungsten | 2. | Silicon or gold |
| 3. | Tungsten or gold | 4. | Selenium or Platinum |
Insertional inactivation of the lac Z gene forms-
| 1. | Blue recombinant colonies |
| 2. | Colourless recombinant colonies |
| 3. | Fluorescent green colonies |
| 4. | There is no relation between the lac Z gene and colour of the colony. |
Two bacteria found to be very useful in genetic engineering are
1. Diplococcus and Streptomyces
2. Rhizobium and Xanthomonas
3. Vibrio and Escherischia
4. Escherischia and Agrobacterium
Which of the following would you choose as the safest and least cumbersome selectable marker-
| 1. | Ampicillin resistance gene |
| 2. | Tetracyclin resistance gene |
| 3. | Kanamycin resistance gene |
| 4. | b Galactosidase gene |
The visualization of the DNA bands on the gel can be done with the help of
1. UV rays
2. Ethidium bromide
3. Chromogenic substrate
4. More than one is correct
Plasmids are good vectors for genetic engineering because
1. They self replicate within bacterial cells
2. Replicate freely outside bacterial cells
3. Can be replicated in culture
4. Can be replicated in laboratory using enzymes
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