Q. No.The underlying basis of the Polymerase Chain Reaction is:
1.
DNA synthesis and hence amplification can occur in a template independent manner.
2.
DNA, being thermostable, can be amplified during the thermal cycle in PCR.
3.
DNA polymerase I can synthesize large amounts of DNA.
4.
PCR allows the direct amplification of DNA even if it is present in minute quantities.
DNA fragments digested by restriction endonucleases separate on agar gel electrophoresis according to their size because:
| 1. | they are forced to move towards cathode under an electric field |
| 2. | agar gel provides a sieving effect |
| 3. | larger fragments will have more negative charge and will move farther in the electric field |
| 4. | chelation with ethidium bromide reduce the rate of transport of fragments |
Plasmid pUC18 carries lacZ gene that confers the host cells to convert white X-gal into blue indole. A gene is inserted within the lacZ gene of the plasmid. Bacterial cells transformed with this rDNA, if provided with X-gal in the medium, will produce:
| 1. | Blue colonies |
| 2. | White colonies |
| 3. | No colonies |
| 4. | Orange colonies if seen through UV light |
Some bacteria are resistant to antibiotics. This trait is normally due to:
1. lack of membrane bound organelles
2. presence of a cell wall
3. presence of an extra-chromosomal circular DNA
4. a unique feature called as inclusion bodies
The downstream processing stage of rDNA does not involve:
| 1. | Biosynthesis | 2. | Separation |
| 3. | Purification | 4. | Preservation |
Which of the following can be used to induce competence for getting a bacterial cell transformed during rDNA procedures?
| 1. | A divalent cation | 2. | Ethidium bromide |
| 3. | Polyethylene glycol | 4. | Anionic proteins |
The Ti plasmid, used in transformation of plant cells, is found in:
| 1. | Agrobacterium tumefaciens | 2. | Escherichia coli |
| 3. | Bacillus thuringiensis | 4. | Xanthomonas citri |
Identify the incorrect statement regarding restriction endonucleases:
| 1. | They mostly cut dsDNA at specific base sequences. |
| 2. | They are produced by bacterial cells as a mechanism of self-defence. |
| 3. | They digest DNA by removing nucleotides from a free 3' end. |
| 4. | They often generate short single-stranded sequences at the ends of the resultant fragments. |
Bioprocessing engineering deals with:
| 1. | the search for plant and animal species from which medicinal drugs and other commercially valuable compounds can be obtained. |
| 2. | developing methods and software tools for understanding biological data. |
| 3. | production of active pharmaceutical substances in genetically modified organisms (GMOs). |
| 4. | maintenance of sterile ambience in chemical engineering processes. |
Agrobacterium tumefaciens can be an effective vector for delivering genes of interest into the cells of:
| 1. | wheat | 2. | soybean |
| 3. | rice | 4. | barley |
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