Q. No.The first type II restriction endonuclease whose functioning depended on a specific DNA nucleotide sequence was:
1.
EcoRI
2.
HindII
3.
SmaI
4.
BamHI
The following palindrome is recognized by the restriction enzyme:
| 1. | BamHI | 2. | EcoRI |
| 3. | HindII | 4. | PstI |
A method used only for transforming animal cells is:
| 1. | Biolistics | 2. | Microinjection |
| 3. | Use of virus | 4. | Agrobacterium mediation |
Lysozyme should be used when isolating DNA in a pure form from:
| 1. | A bacterial cell | 2. | A fungal cell |
| 3. | A plant cell | 4. | An animal cell |
In recombinant DNA experiments, a vector:
| 1. | carries DNA into a new cell |
| 2. | links together newly joined fragments of DN |
| 3. | makes millions of copies of a specific segment of DNA |
| 4. | separates fragments of DNA by their length and electrical charges |
If any protein-encoding gene is expressed in a heterologous host, the protein formed is called:
| 1. | Recombinant protein | 2. | Native protein |
| 3. | Pro-protein | 4. | Exotic protein |
Gene cloning occurs:
| 1. | when a phage transfers bacterial DNA from one bacterium to another. |
| 2. | when a bacterium takes up DNA from the surrounding fluid. |
| 3. | when a bacterium carrying a recombinant plasmid reproduces, thus allowing for the production of multiple copies of the recombinant plasmid. |
| 4. | when DNA is produced from an RNA template. |
Identify (a) and (b) bioreactors respectively in the given diagram:
1. Simple stirred-tank and sparged stirred – tank
2. Sparged stirred - tank and simple stirred-tank
3. Airlift and tower
4. Tower and airlift
A reporter enzyme used in biotechnology includes all the following except:
1. Green Fluorescent Protein
2. Luciferase
3. Beta Galactosidase
4. Chloramphenicol acetyl transferase
What limitation of traditional hybridization procedures used in plant and animal breeding can be overcome by recombinant DNA procedures?
| I: | Inclusion and multiplication of undesirable genes |
| II: | Inability of inclusion of desirable traits from other species |
| 1. | Only I | 2. | Only II |
| 3. | Both I and II | 4. | Neither I nor II |
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