Q. No.What catalyzes the peptide bond formation in prokaryotes?
1. Proteases
2. A ribozyme
3. Sigma subunit of RNA polymerase
4. Rho factor proteins
1. Proteases
2. A ribozyme
3. Sigma subunit of RNA polymerase
4. Rho factor proteins
The genetic material for the first life on the earth was probably:
| 1. | ds DNA | 2. | ss DNA |
| 3. | Protein | 4. | RNA |
Transfer of electrophoresis-separated DNA fragments to a filter membrane and subsequent fragment detection by probe hybridization is called as:
| 1. | DNA profiling | 2. | PCR |
| 3. | Southern blotting | 4. | Elution |
In the human genome, the chromosomes with the most and the fewest genes located on them respectively are:
| 1. | 1 and Y | 2. | X and Y |
| 3. | 21 and X | 4. | 11 and Y |
Avery, MacLeod, and McCarty used DNase in their experiment because:
| 1. | It enabled them to isolate pure DNA samples. |
| 2. | It enabled them to isolate pure protein samples. |
| 3. | This allowed them to demonstrate that removing the DNA prevents transformation. |
| 4. | It made sure that RNA will always be present when they attempted to transform the R strain. |
Which of the following is likely if there is a mutation in the lacY gene of the lac operon in E.coli?
| 1. | The lac genes would be expressed efficiently only in the absence of lactose. |
| 2. | The lac genes would be expressed efficiently until the lactose supply in the cell is exhausted. |
| 3. | The lac genes would be expressed continuously. |
| 4. | Expression of the lac genes would cease immediately. |
Identify the incorrect statement:
| 1. | The DNA of the bacteriophage ϕ X 174 has 5386 nucleotides. |
| 2. | Bacteriophage lambda DNA has 48502 base pairs. |
| 3. | The DNA of Escherichia coli has 4.6 X 103 base pairs. |
| 4. | Haploid content of human DNA is 3.3 X 109 base pairs. |
In eukaryotes, RNA polymerase III transcribes:
| 1. | hnRNA, snRNAs and 5.8S RNA |
| 2. | tRNA, 5.8S RNA and snRNAs |
| 3. | tRNA, 5SrRNA and snRNAs |
| 4. | rRNAs – 28S, 18S and 5S |
Southern blotting is transfer to nitrocellulose sheet from agar gel of:
| 1. | RNA | 2. | Proteins |
| 3. | DNA | 4. | Lipids |
Satellite DNA:
1. Form a small portion of the human genome
2. Code for proteins that are essential for survival
3. Code for proteins that are not essential for survival
4. Show a high degree of polymorphism
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