Q. No.Avery, MacLeod, and McCarty used DNase in their experiment because:
1.
It enabled them to isolate pure DNA samples.
2.
It enabled them to isolate pure protein samples.
3.
This allowed them to demonstrate that removing the DNA prevents transformation.
4.
It made sure that RNA will always be present when they attempted to transform the R strain.
Transfer of electrophoresis-separated DNA fragments to a filter membrane and subsequent fragment detection by probe hybridization is called as:
| 1. | DNA profiling | 2. | PCR |
| 3. | Southern blotting | 4. | Elution |
Which of the following is likely if there is a mutation in the lacY gene of the lac operon in E.coli?
| 1. | The lac genes would be expressed efficiently only in the absence of lactose. |
| 2. | The lac genes would be expressed efficiently until the lactose supply in the cell is exhausted. |
| 3. | The lac genes would be expressed continuously. |
| 4. | Expression of the lac genes would cease immediately. |
Identify the incorrect statement:
| 1. | The DNA of the bacteriophage ϕ X 174 has 5386 nucleotides. |
| 2. | Bacteriophage lambda DNA has 48502 base pairs. |
| 3. | The DNA of Escherichia coli has 4.6 X 103 base pairs. |
| 4. | Haploid content of human DNA is 3.3 X 109 base pairs. |
In eukaryotes, RNA polymerase III transcribes:
| 1. | hnRNA, snRNAs and 5.8S RNA |
| 2. | tRNA, 5.8S RNA and snRNAs |
| 3. | tRNA, 5SrRNA and snRNAs |
| 4. | rRNAs – 28S, 18S and 5S |
Southern blotting is transfer to nitrocellulose sheet from agar gel of:
| 1. | RNA | 2. | Proteins |
| 3. | DNA | 4. | Lipids |
Satellite DNA:
1. Form a small portion of the human genome
2. Code for proteins that are essential for survival
3. Code for proteins that are not essential for survival
4. Show a high degree of polymorphism
The last of 24 human chromosomes to be sequenced was:
1. Chromosome 1 and completed in 2003
2. Chromosome 1 and completed in 2006
3. Chromosome X and completed in 2003
4. Chromosome X and completed in 2006
Identifying all the genes in the genome that are transcribed into RNA is called:
| 1. | Expressed Sequence Tag | 2. | Sequence Annotation |
| 3. | Inverse PCR | 4. | Retrotransposition |
In RNA splicing:
| 1. | Exons are removed and introns are joined together |
| 2. | Introns are removed and exons are joined together |
| 3. | Cistrons are removed and introns are joined together |
| 4. | Introns are removed and Cistrons are joined together |
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