Q. No.What is true regarding A, B and C in the given diagrammatic representation of rDNA technology?
I:
At A, same restriction enzyme is used to cut both foreign and vector DNA
II:
The enzyme used at B is DNA ligase
III:
Step C can be called as transformation
1.
I and II only
2.
I and III only
3.
II and III only
4.
I, II and III
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What is true for the plasmids used as cloning vectors in recombinant procedures?
| 1. | Ori is responsible for controlling the copy number of linked DNA |
| 2. | The bacteria lacking R plasmids are resistant to antibiotics |
| 3. | The vector must have many sites for the same restriction enzyme |
| 4. | They can carry largest inserts amongst the available cloning vectors |
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A and B in pBR322, shown in the diagram given below, respectively represent recognition sequences of:
|
 |
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What is true about the steps of PCR shown in the diagram given below?
| I. | Step A occurs at the temperature of 95 – 980 C |
| II. | Step B occurs at the temperature of 550 C |
| III. | Step C occurs at the temperature of 720 C |
| 1. | I and II only | 2. | I and III only |
| 3. | II and III only | 4. | I, II and III |
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Which of the following primers would allow copying of the single-stranded DNA sequence 5' ATGCCTAGGTC?
| 1. | 5' TACGG | 2. | 5' CTGGA |
| 3. | 5' GACCT | 4. | 5' GGCAT |
The enzyme used in the polymerase chain reaction is a:
1. DNA dependent RNA polymerase
2. RNA dependent DNA polymerase
3. DNA dependent DNA polymerase
4. RNA dependent RNA polymerase
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A host cell normally does not take up a foreign DNA until it has been made competent to do so. This is because:
| 1. | DNA is a hydrophilic molecule |
| 2. | DNA is a very large molecule |
| 3. | there are no receptors for DNA on the cell membrane |
| 4. | DNA is an inert molecule |
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How does the bacterium protects its own DNA from the action of restriction endonucleases present in its cytoplasm?
| 1. | its DNA lacks active sites for the restriction endonucleases |
| 2. | its DNA is protected within a nuclear envelope |
| 3. | it adds methyl groups to the active sites of restriction endonucleases on its DNA |
| 4. | the DNA gets heterochromatised to prevent the action of restriction enzymes |
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When “sticky ends” are paired, they can be joined by:
| 1. | restriction enzymes | 2. | polymerases |
| 3. | methylase | 4. | DNA ligase |
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