1. | Lysozyme or Chitinase | 2. | DNase |
3. | DNAs | 4. | Proteases |
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1. | a complementary DNA sequence synthesised on the template of hnRNA |
2. | artificially synthesised or sequences used to induce replication of DNA |
3. | a single-stranded sequence of DNA or RNA used to search for its complementary sequence in a sample genome |
4. | small chemically synthesised oligonucletides that are complementary to the regions of DNA |
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Assertion (A): | It is possible to select a bacterial cell transformed with a recombinant DNA bearing gene for an antibiotic resistance if the transformed cells are spread on agar plates containing the antibiotic. |
Reason (R): | Due to insertional inactivation of the gene for antibiotic resistance gene, only the untransformed bacterial cells will grow and the transformed bacterial cells will die. |
1. | Both (A) and (R) are False. |
2. | Both (A) and (R) are True and (R) explains (A). |
3. | (A) is True but (R) is False. |
4. | Both (A) and (R) are True but (R) does not explain (A). |
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Assertion (A): | There is a need for the recombinant DNA to be expressed in recombinant DNA technology procedures. |
Reason (R): | In almost all recombinant DNA technologies, the ultimate aim is to produce a desirable protein. |
1. | Both (A) and (R) are False. |
2. | Both (A) and (R) are True and (R) explains (A). |
3. | Both (A) and (R) are True but (R) does not explain (A). |
4. | (A) is True but (R) is False. |
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I: | It is usually spherical in shape. |
II: | The stirrer facilitates even mixing and oxygen availability throughout the reactor. |
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Elution is:
1. | separating the restricted DNA fragments on agarose gel |
2. | staining the separated DNA fragments with ethidium bromide |
3. | cutting out of the separated bands of DNA from the agarose gel and extracting them from the gel piece |
4. | constructing rDNA by joining the purified DNA fragments to the cloning vector |
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A cloning vector has two antibiotic resistance genes: tetracycline and ampicillin. A foreign DNA was inserted into the tetracycline gene. Non-recombinant transformants would survive on the medium containing:
1. | ampicillin but not tetracycline |
2. | tetracycline but not ampicillin |
3. | both tetracycline and ampicillin |
4. | neither tetracycline nor ampicillin |
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Assertion (A): | Bioreactors are important in biotechnology procedures to obtain desired products. |
Reason (R): | Bioreactor provides the optimal conditions for achieving the desired product by providing optimum growth conditions |
1. | Both (A) and (R) are False. |
2. | Both (A) and (R) are True and (R) explains (A). |
3. | Both (A) and (R) are True but (R) does not explain (A). |
4. | (A) is True but (R) is False. |
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Assertion (A): | Agar gel electrophoresis is employed to check the progression of a restriction enzyme digestion. |
Reason (R): | Restriction enzyme digestion is performed by incubating purified DNA molecules with the restriction enzymes, at the optimal conditions for that specific enzyme. |
1. | Both (A) and (R) are False. |
2. | Both (A) and (R) are True but (R) does not explain (A). |
3. | (A) is True but (R) is False. |
4. | Both (A) and (R) are True and (R) explains (A). |
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1. | DNA is the genetic material of all organisms without exception. |
2. | As it is an RNA world, the genetic material is ds RNA in majority of the organisms. |
3. | Since the DNA is enclosed within the membranes, we have to break the cell open to release DNA along with other macromolecules. |
4. | Proteins such as histones can be removed from the cell lysate with the help of ribonuclease. |
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