Q. No.The underlying basis of the Polymerase Chain Reaction is:
1.
DNA synthesis and hence amplification can occur in a template independent manner.
2.
DNA, being thermostable, can be amplified during the thermal cycle in PCR.
3.
DNA polymerase I can synthesize large amounts of DNA.
4.
PCR allows the direct amplification of DNA even if it is present in minute quantities.
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DNA fragments digested by restriction endonucleases separate on agar gel electrophoresis according to their size because:
| 1. | they are forced to move towards cathode under an electric field |
| 2. | agar gel provides a sieving effect |
| 3. | larger fragments will have more negative charge and will move farther in the electric field |
| 4. | chelation with ethidium bromide reduce the rate of transport of fragments |
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Plasmid pUC18 carries lacZ gene that confers the host cells to convert white X-gal into blue indole. A gene is inserted within the lacZ gene of the plasmid. Bacterial cells transformed with this rDNA, if provided with X-gal in the medium, will produce:
| 1. | Blue colonies |
| 2. | White colonies |
| 3. | No colonies |
| 4. | Orange colonies if seen through UV light |
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A cloning vector must:
| 1. | be able to maintain a very high copy numbers of their genome in the host cells |
| 2. | contain elements such as promoter and ribosome binding sites |
| 3. | be engineered to carry a large number of recognition sequences for common restriction enzymes |
| 4. | be able to replicate within host cell independent of the control of chromosomal DNA |
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Some bacteria are resistant to antibiotics. This trait is normally due to:
1. lack of membrane bound organelles
2. presence of a cell wall
3. presence of an extra-chromosomal circular DNA
4. a unique feature called as inclusion bodies
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The downstream processing stage of rDNA does not involve:
| 1. | Biosynthesis | 2. | Separation |
| 3. | Purification | 4. | Preservation |
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In microinjection, the rDNA :
| 1. | coats the micropellets and is used in biolistics to transform plant cells |
| 2. | is directly injected into the cytoplasm of a plant or an animal cell |
| 3. | is delivered in the vicinity of the plasma membrane of the cell (to be transformed) |
| 4. | is directly injected into the nucleus of an animal cell |
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Which of the following can be used to induce competence for getting a bacterial cell transformed during rDNA procedures?
| 1. | A divalent cation | 2. | Ethidium bromide |
| 3. | Polyethylene glycol | 4. | Anionic proteins |
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The Ti plasmid, used in transformation of plant cells, is found in:
| 1. | Agrobacterium tumefaciens | 2. | Escherichia coli |
| 3. | Bacillus thuringiensis | 4. | Xanthomonas citri |
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If we are able to link an alien piece of DNA with bacteriophage or plasmid DNA, we can multiply its number:
| 1. | more than the number of copy number of bacteriophages in the cells and equal to the copy number of plasmid in the cell. |
| 2. | equal to the number of copy number of bacteriophages in the cells and equal to the copy number of plasmid in the cell. |
| 3. | equal to the number of copy number of bacteriophages in the cells and less than the copy number of plasmid in the cell. |
| 4. | less than the number of copy number of bacteriophages in the cells and more than the copy number of plasmid in the cell. |
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