Q. No.There is a restriction endonuclease called EcoRI. What does ‘co’ part in it stand for?
1. Colon
2. Coelom
3. Coenzyme
4. coli
1. Colon
2. Coelom
3. Coenzyme
4. coli
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Agarose extracted from sea weeds finds use in
1. Spectrophotometry
2. Tissue Culture
3. PCR
4. Gel electrophoresis
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Which one of the following techniques made it possible to genetically engineer living organisms?
1. Recombinant DNA techniques
2. X-ray diffraction
3. Heavier isotope labeling
4. Hybridization
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The figure below shows three steps (A, B, C) of Polymerase Chain Reaction (PCR). Select the option giving correct identification together with what it represents?
Options:
1. B - Denaturation at a temperature of about 98°C separating the two DNA strands.
2. A - Denaturation at a temperature of about 50°C
3. C - Extension in the presence of heat-stable DNA polymerase
4. A - Annealing with two sets of primers
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Which one of the following represents a palindromic sequence in DNA?
1. 5' - GAATTC - 3'
3' - CTTAAG - 5'
2. 5' - CCAATG - 3'
3' - GAATCC - 5'
3. 5' - CATTAG - 3'
3' - GATAAC - 5'
4. 5' - GATACC - 3'
3' - CCTAAG - 5'
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DNA fragments generated by the restriction endonucleases in a chemical reaction can be separated by:
1. Polymerase chain reaction
2. Electrophoresis
3. Restriction mapping
4. Centrifugation
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Restriction endonuclease
1. Cuts the DNA molecule randomly
2. Cuts the DNA molecule at specific sites
3. Restricts the synthesis of DNA inside the nucleus.
4. Synthesizes DNA.
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Two microbes found to be very useful in gene engineering are
1. Escherichia coli and Agrobacterium tumefaciens.
2. Vibrio cholerae and a tailed bacteriophage.
3. Diplococcus sp. And Pseudomonas sp.
4. Crown gall bacterium and Caenorphaditiselegans.
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Gel electrophoresis is used for
1. Isolation of DNA molecules.
2. Cutting of DNA into fragments.
3. Separation of DNA fragments according to their size.
4. Construction of recombinant DNA by joining with cloning vector.
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Polyethylene glycol method is used for
1. Energy production from sewage.
2. Gene transfer without a vector
3. Biodiesel production.
4. Seedless fruit production.
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