Which of the following should be chosen for the best yield if one were to produce a recombinant protein in large amounts?
1. | Laboratory flask of the largest capacity |
2. | A stirred-tank bioreactor without in-lets and out-lets |
3. | A continuous culture system |
4. | Any of the above |
For the multiplication of any alien piece of DNA in an organism, it needs to be part of a chromosome that has a specific:
1. | Telomeric sequence | 2. | Multiple cloning sites |
3. | Ori | 4. | Selectable marker |
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The first type II restriction endonuclease whose functioning depended on a specific DNA nucleotide sequence was:
1. | EcoRI | 2. | HindII |
3. | SmaI | 4. | BamHI |
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The following palindrome is recognized by the restriction enzyme:
1. | BamHI | 2. | EcoRI |
3. | HindII | 4. | PstI |
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A method used only for transforming animal cells is:
1. | Biolistics | 2. | Microinjection |
3. | Use of virus | 4. | Agrobacterium mediation |
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Lysozyme should be used when isolating DNA in a pure form from:
1. | A bacterial cell | 2. | A fungal cell |
3. | A plant cell | 4. | An animal cell |
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In recombinant DNA experiments, a vector:
1. | carries DNA into a new cell |
2. | links together newly joined fragments of DN |
3. | makes millions of copies of a specific segment of DNA |
4. | separates fragments of DNA by their length and electrical charges |
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If any protein-encoding gene is expressed in a heterologous host, the protein formed is called:
1. | Recombinant protein | 2. | Native protein |
3. | Pro-protein | 4. | Exotic protein |
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Gene cloning occurs:
1. | when a phage transfers bacterial DNA from one bacterium to another. |
2. | when a bacterium takes up DNA from the surrounding fluid. |
3. | when a bacterium carrying a recombinant plasmid reproduces, thus allowing for the production of multiple copies of the recombinant plasmid. |
4. | when DNA is produced from an RNA template. |
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Identify (a) and (b) bioreactors respectively in the given diagram:
1. Simple stirred-tank and sparged stirred – tank
2. Sparged stirred - tank and simple stirred-tank
3. Airlift and tower
4. Tower and airlift
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