Q. No.Which of the following is not required in the preparation of a recombinant DNA molecule?
1.
Restriction endonuclease
2.
DNA ligase
3.
DNA fragments
4.
E.coli
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The most important feature in a plasmid to serve as a vector in gene cloning experiment is:
1. Origin of replication (ori)
2. Presence of a selectable marker
3. Presence of sites for restriction endonuclease
4. Its size
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While isolating DNA from bacteria, which of the following enzymes is not required?
| 1. | Lysozyme | 2. | Ribonuclease |
| 3. | Deoxyribonuclease | 4. | Protease |
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Which of the following contributed in popularising the PCR (polymerase chain reactions) technique?
| 1. | Easy availability of DNA template |
| 2. | Availability of synthetic primers |
| 3. | Availability of cheap deoxyribonucleotides |
| 4. | Availability of 'Thermostable' DNA polymerase |
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Which of the following steps are catalysed by Taq DNA polymerase in a PCR reaction?
1. Denaturation of template DNA
2. Annealing of primers to template DNA
3. Extension of primer end on the template DNA
4. All of the above
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Which of the following should be chosen for the best yield if one were to produce a recombinant protein in large amounts?
| 1. | Laboratory flask of the largest capacity |
| 2. | A stirred-tank bioreactor without in-lets and out-lets |
| 3. | A continuous culture system |
| 4. | Any of the above |
Which of the following statements does not hold true for restriction enzyme?
| 1. | It recognises a palindromic nucleotide sequence |
| 2. | It is an endonuclease |
| 3. | It is isolated from viruses |
| 4. | It can produce the same kind of sticky ends in different DNA molecules |
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Restriction endonucleases present in bacterial cells provide an advantage to the cell because these enzymes:
| 1. | can identify the mutated sequences on the chromosomal DNA and help in their repair |
| 2. | selectively bind to particular nucleotide sequences that may appear in viral DNA preventing its replication in the cell |
| 3. | can cut the plasmid DNA of the bacterial cell that allows them to recombine foreign DNA into their plasmid DNA |
| 4. | help the bacterium take up foreign DNA from their environment and combine it into their genome |
For the multiplication of any alien piece of DNA in an organism, it needs to be part of a chromosome that has a specific:
| 1. | Telomeric sequence | 2. | Multiple cloning sites |
| 3. | Ori | 4. | Selectable marker |
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The first type II restriction endonuclease whose functioning depended on a specific DNA nucleotide sequence was:
| 1. | EcoRI | 2. | HindII |
| 3. | SmaI | 4. | BamHI |
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