Which of the given statements is correct in the context of visualizing DNA molecules separated by agarose gel electrophoresis?
1. | DNA can be seen in visible light |
2. | DNA can be seen without staining in visible light |
3. | Ethidium bromide stained DNA can be seen in visible light |
4. | Ethidium bromide stained DNA can be seen under exposure to UV light |
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'Restriction' in Restriction enzyme refers to:
1. | Cleaving of the phosphodiester bonds in DNA by the enzyme |
2. | Cutting of DNA at a specific position only |
3. | Prevention of the multiplication of bacteriophage by the host bacteria |
4. | All of the above |
The most important feature in a plasmid to serve as a vector in gene cloning experiment is:
1. Origin of replication (ori)
2. Presence of a selectable marker
3. Presence of sites for restriction endonuclease
4. Its size
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Which of the following steps are catalysed by Taq DNA polymerase in a PCR reaction?
1. Denaturation of template DNA
2. Annealing of primers to template DNA
3. Extension of primer end on the template DNA
4. All of the above
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Which of the following should be chosen for the best yield if one were to produce a recombinant protein in large amounts?
1. | Laboratory flask of the largest capacity |
2. | A stirred-tank bioreactor without in-lets and out-lets |
3. | A continuous culture system |
4. | Any of the above |
For the multiplication of any alien piece of DNA in an organism, it needs to be part of a chromosome that has a specific:
1. | Telomeric sequence | 2. | Multiple cloning sites |
3. | Ori | 4. | Selectable marker |
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The first type II restriction endonuclease whose functioning depended on a specific DNA nucleotide sequence was:
1. | EcoRI | 2. | HindII |
3. | SmaI | 4. | BamHI |
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The following palindrome is recognized by the restriction enzyme:
1. | BamHI | 2. | EcoRI |
3. | HindII | 4. | PstI |
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A method used only for transforming animal cells is:
1. | Biolistics | 2. | Microinjection |
3. | Use of virus | 4. | Agrobacterium mediation |
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Lysozyme should be used when isolating DNA in a pure form from:
1. | A bacterial cell | 2. | A fungal cell |
3. | A plant cell | 4. | An animal cell |
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