The transfer of genetic material from one bacterium to another through the mediation of a viral vector is termed as:
1. | Transduction | 2. | Conjugation |
3. | Transformation | 4. | Translation |
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In agarose gel electrophoresis, DNA molecules are separated on the basis of their:
1. | Charge only | 2. | Size only |
3. | Charge-to-size ratio | 4. | All of the above |
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An antibiotic resistance gene in a vector usually helps in the selection of:
1. Competent bacterial cells
2. Transformed bacterial cells
3. Recombinant bacterial cells
4. None of the above
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The significance of the 'heat shock' method in bacterial transformation is to facilitate:
1. | Binding of DNA to the cell wall |
2. | Uptake of DNA through membrane transport proteins |
3. | Uptake of DNA through transient pores in the bacterial cell wall |
4. | Expression of antibiotic resistance gene |
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A bacterial cell was transformed with a recombinant DNA molecule that was generated using a human gene. However, the transformed cells did not produce the desired protein. The reasons could be:
1. | Human genes may have intron which bacteria cannot process |
2. | Amino acid codons for humans and bacteria are different |
3. | Human protein is formed but degraded by bacteria |
4. | All of the above |
Who among the following was awarded the Nobel Prize for the development of the PCR technique?
1. | Herbert Boyer | 2. | Hargovind Khurana |
3. | Kary Mullis | 4. | Arthur Kornberg |
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Which of the following cannot be used as a vector in rDNA technology?
1. | Plasmid | 2. | Phage DNA |
3. | Bacterium | 4. | YAC |
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Which of the following does not have the ability to replicate within bacterial cells independent of the control of chromosomal DNA?
1. | Plastid | 2. | Bacteriophages |
3. | BAC | 4. | Plasmid |
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It is useful to use restriction enzymes in rDNA experiments that produce sticky ends in the resultant fragments because:
1. | it allows a cell to recognize fragments produced by the enzyme |
2. | the single-stranded ends serve as starting points for DNA replication |
3. | the fragments will bond to other fragments with complementary single-stranded ends |
4. | only single-stranded DNA segments can code for proteins |
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Restriction enzymes do not act on the DNA of the host cell in which they are produced, because :
1. | Host DNA is packed into chromosomes |
2. | Restriction enzymes are ineffective on host DNA |
3. | Host DNA does not have the restriction site for the restriction enzymes. |
4. | Restriction site of host DNA is methylated. |
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