If the required gene has been isolated by shotgun approach, care must be taken to:
1. | cut the plasmid with a different restriction enzyme than that used to obtain the donor gene |
2. | cut the plasmid with the same restriction enzyme that was used to obtain the donor gene |
3. | avoid cutting the plasmid |
4. | cut the plasmid with two different restriction enzymes |
To unlock all the explanations of 38 chapters you need to be enrolled in MasterClass Course.
To unlock all the explanations of 38 chapters you need to be enrolled in MasterClass Course.
The chronological steps of a polymerase chain reaction are:
1. | Denaturation, Annealing of primers, Primer extension |
2. | Annealing of primers, Denaturation, Primer extension |
3. | Annealing of primers, Primer extension, Denaturation |
4. | Denaturation, Primer extension, Annealing of primers |
To unlock all the explanations of 38 chapters you need to be enrolled in MasterClass Course.
To unlock all the explanations of 38 chapters you need to be enrolled in MasterClass Course.
Taq polymerase is used in the polymerase chain reaction because:
1. | It replicates DNA faster than other enzymes |
2. | It is the only enzyme that can replicate DNA invitro |
3. | It does not require an RNA primer to function |
4. | It is thermostable |
To unlock all the explanations of 38 chapters you need to be enrolled in MasterClass Course.
To unlock all the explanations of 38 chapters you need to be enrolled in MasterClass Course.
A major advantage of using YAC as a cloning vector over the plasmids is that:
1. it can replicate independently
2. it can be selected easily
3. it can accommodate larger inserts
4. it has multiple cloning sites
Which of the following enzymes catalyze the removal of nucleotides from the ends of DNA?
1. | endonuclease | 2. | exonuclease |
3. | DNA ligase | 4. | Hind – II |
To unlock all the explanations of 38 chapters you need to be enrolled in MasterClass Course.
To unlock all the explanations of 38 chapters you need to be enrolled in MasterClass Course.
The transfer of genetic material from one bacterium to another through the mediation of a viral vector is termed as:
1. | Transduction | 2. | Conjugation |
3. | Transformation | 4. | Translation |
To unlock all the explanations of 38 chapters you need to be enrolled in MasterClass Course.
To unlock all the explanations of 38 chapters you need to be enrolled in MasterClass Course.
In agarose gel electrophoresis, DNA molecules are separated on the basis of their:
1. | Charge only | 2. | Size only |
3. | Charge-to-size ratio | 4. | All of the above |
To unlock all the explanations of 38 chapters you need to be enrolled in MasterClass Course.
To unlock all the explanations of 38 chapters you need to be enrolled in MasterClass Course.
An antibiotic resistance gene in a vector usually helps in the selection of:
1. Competent bacterial cells
2. Transformed bacterial cells
3. Recombinant bacterial cells
4. None of the above
To unlock all the explanations of 38 chapters you need to be enrolled in MasterClass Course.
To unlock all the explanations of 38 chapters you need to be enrolled in MasterClass Course.
The significance of the 'heat shock' method in bacterial transformation is to facilitate:
1. | Binding of DNA to the cell wall |
2. | Uptake of DNA through membrane transport proteins |
3. | Uptake of DNA through transient pores in the bacterial cell wall |
4. | Expression of antibiotic resistance gene |
To unlock all the explanations of 38 chapters you need to be enrolled in MasterClass Course.
To unlock all the explanations of 38 chapters you need to be enrolled in MasterClass Course.
A bacterial cell was transformed with a recombinant DNA molecule that was generated using a human gene. However, the transformed cells did not produce the desired protein. The reasons could be:
1. | Human genes may have intron which bacteria cannot process |
2. | Amino acid codons for humans and bacteria are different |
3. | Human protein is formed but degraded by bacteria |
4. | All of the above |