How does the bacterium protects its own DNA from the action of restriction endonucleases present in its cytoplasm?
1. | its DNA lacks active sites for the restriction endonucleases |
2. | its DNA is protected within a nuclear envelope |
3. | it adds methyl groups to the active sites of restriction endonucleases on its DNA |
4. | the DNA gets heterochromatised to prevent the action of restriction enzymes |
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When “sticky ends” are paired, they can be joined by:
1. | restriction enzymes | 2. | polymerases |
3. | methylase | 4. | DNA ligase |
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Which of the following is not a plasmid?
1. | pSC101 | 2. | pUC18 |
3. | BamHI | 4. | pBR322 |
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Which of the following is normally not used as a method of introduction of foreign DNA into an animal cell?
1. | Microinjection | 2. | Liposomes |
3. | Biolistics | 4. | Transfection |
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Which of the given statements is correct in the context of visualizing DNA molecules separated by agarose gel electrophoresis?
1. | DNA can be seen in visible light |
2. | DNA can be seen without staining in visible light |
3. | Ethidium bromide stained DNA can be seen in visible light |
4. | Ethidium bromide stained DNA can be seen under exposure to UV light |
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'Restriction' in Restriction enzyme refers to:
1. | Cleaving of the phosphodiester bonds in DNA by the enzyme |
2. | Cutting of DNA at a specific position only |
3. | Prevention of the multiplication of bacteriophage by the host bacteria |
4. | All of the above |
Which of the following is not required in the preparation of a recombinant DNA molecule?
1. | Restriction endonuclease | 2. | DNA ligase |
3. | DNA fragments | 4. | E.coli |
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The most important feature in a plasmid to serve as a vector in gene cloning experiment is:
1. Origin of replication (ori)
2. Presence of a selectable marker
3. Presence of sites for restriction endonuclease
4. Its size
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While isolating DNA from bacteria, which of the following enzymes is not required?
1. | Lysozyme | 2. | Ribonuclease |
3. | Deoxyribonuclease | 4. | Protease |
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Which of the following contributed in popularising the PCR (polymerase chain reactions) technique?
1. | Easy availability of DNA template |
2. | Availability of synthetic primers |
3. | Availability of cheap deoxyribonucleotides |
4. | Availability of 'Thermostable' DNA polymerase |
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