Q. No.How does the bacterium protects its own DNA from the action of restriction endonucleases present in its cytoplasm?
1.
its DNA lacks active sites for the restriction endonucleases
2.
its DNA is protected within a nuclear envelope
3.
it adds methyl groups to the active sites of restriction endonucleases on its DNA
4.
the DNA gets heterochromatised to prevent the action of restriction enzymes
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When “sticky ends” are paired, they can be joined by:
| 1. | restriction enzymes | 2. | polymerases |
| 3. | methylase | 4. | DNA ligase |
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Which of the following is normally not used as a method of introduction of foreign DNA into an animal cell?
| 1. | Microinjection | 2. | Liposomes |
| 3. | Biolistics | 4. | Transfection |
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Which of the given statements is correct in the context of visualizing DNA molecules separated by agarose gel electrophoresis?
| 1. | DNA can be seen in visible light |
| 2. | DNA can be seen without staining in visible light |
| 3. | Ethidium bromide stained DNA can be seen in visible light |
| 4. | Ethidium bromide stained DNA can be seen under exposure to UV light |
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'Restriction' in Restriction enzyme refers to:
| 1. | Cleaving of the phosphodiester bonds in DNA by the enzyme |
| 2. | Cutting of DNA at a specific position only |
| 3. | Prevention of the multiplication of bacteriophage by the host bacteria |
| 4. | All of the above |
Which of the following is not required in the preparation of a recombinant DNA molecule?
| 1. | Restriction endonuclease | 2. | DNA ligase |
| 3. | DNA fragments | 4. | E.coli |
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The most important feature in a plasmid to serve as a vector in gene cloning experiment is:
1. Origin of replication (ori)
2. Presence of a selectable marker
3. Presence of sites for restriction endonuclease
4. Its size
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While isolating DNA from bacteria, which of the following enzymes is not required?
| 1. | Lysozyme | 2. | Ribonuclease |
| 3. | Deoxyribonuclease | 4. | Protease |
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Which of the following contributed in popularising the PCR (polymerase chain reactions) technique?
| 1. | Easy availability of DNA template |
| 2. | Availability of synthetic primers |
| 3. | Availability of cheap deoxyribonucleotides |
| 4. | Availability of 'Thermostable' DNA polymerase |
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