Q. No.A host cell normally does not take up a foreign DNA until it has been made competent to do so. This is because:
1.
DNA is a hydrophilic molecule
2.
DNA is a very large molecule
3.
there are no receptors for DNA on the cell membrane
4.
DNA is an inert molecule
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How does the bacterium protects its own DNA from the action of restriction endonucleases present in its cytoplasm?
| 1. | its DNA lacks active sites for the restriction endonucleases |
| 2. | its DNA is protected within a nuclear envelope |
| 3. | it adds methyl groups to the active sites of restriction endonucleases on its DNA |
| 4. | the DNA gets heterochromatised to prevent the action of restriction enzymes |
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When “sticky ends” are paired, they can be joined by:
| 1. | restriction enzymes | 2. | polymerases |
| 3. | methylase | 4. | DNA ligase |
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Which of the following is normally not used as a method of introduction of foreign DNA into an animal cell?
| 1. | Microinjection | 2. | Liposomes |
| 3. | Biolistics | 4. | Transfection |
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Which of the given statements is correct in the context of visualizing DNA molecules separated by agarose gel electrophoresis?
| 1. | DNA can be seen in visible light |
| 2. | DNA can be seen without staining in visible light |
| 3. | Ethidium bromide stained DNA can be seen in visible light |
| 4. | Ethidium bromide stained DNA can be seen under exposure to UV light |
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'Restriction' in Restriction enzyme refers to:
| 1. | Cleaving of the phosphodiester bonds in DNA by the enzyme |
| 2. | Cutting of DNA at a specific position only |
| 3. | Prevention of the multiplication of bacteriophage by the host bacteria |
| 4. | All of the above |
Which of the following is not required in the preparation of a recombinant DNA molecule?
| 1. | Restriction endonuclease | 2. | DNA ligase |
| 3. | DNA fragments | 4. | E.coli |
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The most important feature in a plasmid to serve as a vector in gene cloning experiment is:
1. Origin of replication (ori)
2. Presence of a selectable marker
3. Presence of sites for restriction endonuclease
4. Its size
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While isolating DNA from bacteria, which of the following enzymes is not required?
| 1. | Lysozyme | 2. | Ribonuclease |
| 3. | Deoxyribonuclease | 4. | Protease |
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