Identify the correct statement:

1.	The structural gene is polycistronic mostly in eukaryotes and monocistronic mostly in prokaryotes.
2.	Introns are said to be those sequences in the eukaryotic structural gene that appear in mature or processed mRNA.
3.	During transcription both strands of the DNA are transcribed but in a short length.
4.	Transcription and translation can be coupled in bacteria.

What is added during ‘capping’ in post-transcriptional modification of hn RNA?
1. Methyl guanosine triphosphate at 5’ end
2. Methyl guanosine triphosphate at 3’ end
3. Adenylate residues at the 5’ end
4. Adenylate residues at the 3’ end

Name the enzyme that facilitates opening of DNA helix during transcription.

1. DNA helicase

2. DNA polymerase

3. RNA polymerase

4. DNA ligase

Choose the correct pair from the following :

During DNA replication the leading strand:
1. is replicated continuously towards the replication fork
2. is replicated discontinuously towards the replication fork
3. is replicated continuously away from the replication fork
4. is replicated continuously away from the replication fork

Transfer of electrophoresis-separated DNA fragments to a filter membrane and subsequent fragment detection by probe hybridization is called as:

The template strand of DNA is 3’ – TAC – 5’. The anticodon that will pair the corresponding mRNA codon will be:
1. 5’ – AUG – 3’
2. 5’ – UAC – 3’
3. 5’ – CAU – 3’
4. 3’ – TAC – 5’

How many recognition sequences for the restriction enzyme EcoRI is/are expected to be present in a dsDNA of 10000 base pair length?
1. 0
2. 1
3. 2
4. 4

In eukaryotes, RNA polymerase II transcribes:
1. heterogenous nuclear RNA
2. small nuclear RNA
3. transfer RNA
4. ribosomal RNA

During translation, formation of peptide bonds in bacteria is catalyzed by:
1. Polynucleotide phosphorylase
2. Protease
3. Ligase
4. Ribozyme