Q. No.What is an antisense technology?
(1) A cell displaying a foreign antigen used for synthesis of antigens.
(2) Production of somaclonal variants in tissue cultures.
(3) When a piece of RNA that is complementary in sequence is used to stop expression of a specific gene
(4) RNA polymerase producing DNA
Cultivation of Bt cotton has been much in the news. The prefix Bt means
(1) "Barium-treated" cotton seeds
(2) "Bigger thread" variety of cotton with better tensile strength
(3) produced by "biotechnology" using restriction enzymes and ligases
(4) carrying an endotoxin gene from Bacillus thuringienesis.
RNA interference is essential for the
(1) cell proliferation
(2) cell defence
(3) cell differentiation
(4) micropropagation
Genetically modified (GM) crops can be produced by
(1) recombinant DNA technology
(2) somatic hybridization
(3) cross breeding
(4) micropropagation
Which of the following genes do not occur naturally in living organisms?
(1) Bt genes
(2) RNAi genes
(3) Cry genes
(4) Endogeneous cytoplasmic defense genes
The protein products of the following Bt toxin genes cry I Ac and cry II Ab are responsible for controlling
(1) bollworm
(2) roundworm
(3) moth
(4) fruit fly
Three critical research areas of biotechnology are:
I. Providing the best catalyst in the form of improved organism usually a microbe or pure enzyme
II. Multiple Ovulation Transfer Technology (MOET)
III. Creating optimal conditions through engineering for a catalyst to act.
IV. Downstream processing technologies
(1) I, II, III, IV
(2) I, III, IV
(3) I, II
(4) II and IV
Bt toxin is harmful to insects like:
(1) Lepidoterans (tobacco budworm, armyworms)
(2) Coleopterans (beetles)
(3) Dipterans (flies and mosquito)
(4) All are correct
Which of the following statements is false?
| I. | Insulin was originally extracted from pancreas of slaughtered pigs and cattle |
| II. | Animal insulin is difficult to obtain |
| III. | Animal insulin is identical to human insulin |
| IV. | Non-human insulin caused some patients to develop allergy |
| V. | Recombinant DNA allowed scientists to insert a human insulin gene into a bacterial expression vector |
| VI. | Recombinant insulin is actually obtained from E. coli bacterial cell |
1. Only I and II
2. Only III and IV
3. Only III
4. Only VI
Which step proved to be the main challenging obstacle in the production of human insulin by genetic engineering?
(1) Splitting A and B polypeptide chains
(2) Addition of C-peptide to pro-insulin
(3) Getting insulin assembled into mature form
(4) Removal of C-peptide from active insulin
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