Consider the following steps involved in recombinant DNA technology 
A. Downstream processing 
B. Insertion of recombinant DNA into host cell 
C. Isolation of genetic material 
D. Obtaining the foreign gene product 
E. Cutting of DNA at specific locations 
Choose the correct sequence of steps 
1. C, A, B, D, E 
2. C, E, B, D, A 
3. A, E, B, D, C
4. C, E, D, B, A 
Subtopic:  General Design of an rDNA experiment |
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Match List I with List II:
List I List II
A. Chloramphenicol I. Join the cut ends of DNA molecule
B. Ethidium bromide II. Makes bacterial cell competent to take up DNA
C. DNA ligase III. Selectable marker
D. Calcium IV. Agarose gel staining
Choose the correct answer from the options given below:
1.  A - (III); B - (IV); C- (II); D - (I)
2.  A - (III); B - (IV); C- (I); D - (II)
3.  A - (IV); B - (III); C- (II); D - (I)
4.  A - (IV); B - (III); C- (I); D - (II)
Subtopic:  General Design of an rDNA experiment |
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Read the following statements and choose the correct option.
Statement (A): In gel electrophoresis, the most commonly used matrix is agar.
Statement (B): Agar is a natural polymer extracted from sea weeds.
1. Both statements (A) and (B) are correct
2. Both statements (A) and (B) are incorrect
3. Only statement (A) is correct
4. Only statement (B) is correct
Subtopic:  Separation and Isolation of DNA fragments |
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How many statements given below are correct with respect to plasmids?
a. They float freely in the cytoplasm of certain bacterial cells.
b. They have the ability to replicate, independent of the genomic DNA.
c. Herbert Boyer developed a method of removing and reinserting them in the cells.
d. They are circular ssDNA molecules.
Select the correct option.
1. Three
2. Four
3. Two
4. One
Subtopic:  Introduction & History |
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Which of the following can act as selectable markers in a cloning vector?
a. ampR
b. tetR
c. ori
d. rop
Select the correct option.
1. (a) and (b)
2. (c) and (d)
3. (a), (c) and (d)
4. (a), (b), (c) and (d)
Subtopic:  Cloning Vector |
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Match column I with column II and choose the correct option:
Column I Column II
a. Salmonella typhimurium (i) Source of EcoRI
b. Bacillus thuringiensis (ii) Used in developing transgenic tobacco plant
c. Agrobacterium
tumefaciens
(iii) Used in creating first
recombinant DNA
d. Escherichia coli (iv) Gene used in creating biopesticide

1. a(iv), b(i), c(ii), d(iii)
2. a(iii), b(iv), c(i), d(ii)
3. a(i), b(iv), c(ii), d(iii)
4. a(iii), b(iv), c(ii), d(i)
Subtopic:  Restriction Enzymes - Main Enzymes | Transforming Plant & Animal Cell | Restriction Enzymes: Historical Background |
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In agarose gel electrophoresis, DNA molecules are separated on the basis of
a. Size
b. Charge
c. Charge to size ratio
d. Length of agarose gel
Select the correct option:
1. a only
2. b only
3. a, b and c
4. a, b, c and d
Subtopic:  Separation and Isolation of DNA fragments |
 78%
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Complete the analogy and select the correct option.
BamHI : Sticky ends : : EcoRV : ______
1. Staggered ends
2. Cohesive ends
3. Blunt ends
4. Ends with overhanging stretches
Subtopic:  Restriction Enzymes - Main Enzymes |
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Assertion (A): If BamHI cuts a 12 kb linear dsDNA fragment at two restriction sites which are at the same distance from the ends and each other, then only one band is obtained on agarose gel electrophoresis.
Reason (R): Three equal sized fragments will be obtained on restriction digestion by BamHI.
In the light of above statements, choose the correct answer from the following options.
1. Both (A) and (R) are true but (R) is not the correct explanation of (A)
2. Both (A) and (R) are true and (R) is the correct explanation of (A)
3. (A) is false but (R) is true
4. (A) is true but (R) is false
Subtopic:  Tools: Enzymes: I |
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Match column-I with column-II and select the correct option:
Column I Column II
a. Restriction endonuclease (i) Hind II
b. First recombinant DNA (ii) Obtained from
strain RY13
c. First restriction endonuclease (iii) Isolated in 1963
d. EcoRI (iv) Developed in 1972

1. a(iii), b(i), c(ii), d(iv)
2. a(i), b(ii), c(iii), d(iv)
3. a(iii), b(iv), c(i), d(ii)
4. a(iii), b(iv), c(ii), d(i)
Subtopic:  Restriction Enzymes: Historical Background | Introduction & History |
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