Which of the following enzymes catalyse the removal of nucleotides from the ends of DNA?
1. endonuclease     
2. exonuclease       
3. DNA ligase         
4. Hind - Il

Subtopic:  Restriction Enzymes - Main Enzymes |
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The transfer of genetic material from one bacterium to another through the mediation of a viral vector is termed as
1. transduction     
2. conjugation     
3.  transformation         
4. translation

Subtopic:  Transforming Plant & Animal Cell |
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Which of the given statement is correct in the context of visualising DNA molecules separated by agarose gel electrophoresis?
 

1. DNA can be seen in visible light.
2. DNA can be seen without staining in visible light.
3. Ethidium bromide stained DNA can be seen in visible light
4. Ethidium bromide stained DNA can be seen under exposure to UV light.

Subtopic:  Separation and Isolation of DNA fragments |
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‘Restriction’ in restriction enzyme refers to

1. cleaving of phosphodiester bond in DNA by the enzyme
2. cutting of DNA at specific position only
3. prevention of the multiplication of bacteriophage by the host bacteria
4. All of the above


 

Subtopic:  Restriction Enzymes - Main Enzymes |
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Which of the following is not required in the preparation of a recombinant DNA molecule?
1. Restriction endonuclease                   
2. DNA ligase
3. DNA fragments                                   
4. E. coli

Subtopic:  General Design of an rDNA experiment |
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In agarose gel electrophoresis, DNA molecules are separated on the basis of their

1. charge only       
2. size only     
3. charge to size ratio       
4. All of the above

Subtopic:  Separation and Isolation of DNA fragments |
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The most important feature in a plasmid to serve as a vector in gene cloning experiment is:
1. Origin of replication (ori)
2. Presence of a selectable marker
3. Presence of sites for restriction endonuclease
4. Its size

Subtopic:  Cloning Vector |
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While isolating DNA from bacteria, which of the following enzymes is not required?
1. Lysozyme                             
2. Ribonuclease
3. Deoxyribonuclease                 
4. Protease

Subtopic:  Process of Biotech | Obtaining Copy of Gene from Donor DNA | Host & Desired DNA |
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Which of the following has popularised the PCR (Polymerase Chain Reaction)?
1. Easy availability of DNA template
2. Availability of synthetic primers.
3. Availability of cheap deoxyribonucleotides
4. Availability of ‘Thermostable’ DNA polymerase

Subtopic:  Polymerase Chain Reaction: PCR |
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An antibiotic resistance gene in a vector usually helps in the selection of

1. Competent bacterial cells                             
2. Transformed bacterial cells
3. Recombinant bacterial cells                           
4. None of the above

Subtopic:  Selection of Recombinant Transformants |
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