Upon exposure to UV radiation, DNA stained with ethidium bromide will show: 
1.Bright orange colour2.Bright red colour
3.Bright Blue colour4.Bright yellow colour
Subtopic:  Separation and Isolation of DNA fragments |
 90%
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NEET - 2023
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During the purification process for recombinant DNA technology, addition of chilled ethanol precipitates out:
1. Polysaccharides  2. RNA 
3. DNA  4. Histones 
Subtopic:  General Design of an rDNA experiment |
 86%
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NEET - 2023
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In gene gun method used to introduce alien DNA into host cells, microparticles of ___________ metal are used. 
1.Silver 2.Copper 
3.Zinc 4.Tungsten or gold
Subtopic:  Transforming Plant & Animal Cell |
 91%
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NEET - 2023
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Main steps in the formation of Recombinant DNA are given below. Arrange these steps in a correct sequence.
 
A. Insertion of recombinant DNA into the host cell.
B. Cutting of DNA at specific location by restriction enzyme.
C. Isolation of desired DNA fragment.
D. Amplification of gene of interest using PCR.

Choose the correct answer from the options given below:
1.B, D, A, C2.B, C, D, A
3.C, A, B, D4.C, B, D, A
Subtopic:  Process of Biotech |
From NCERT
NEET - 2023
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Thermostable DNA polymerase used in PCR was isolated from:
1. Thermus aquaticus
2. Escherichia coli
3. Agrobacterium tumefaciens
4. Bacillus thuringiensis
Subtopic:  Polymerase Chain Reaction: PCR |
 89%
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NEET - 2023
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Ligation of foreign DNA at which of the following site will result in loss of tetracycline resistance of pBR322?
1. PsT I
2. Pvu I
3. EcoR I
4. BamH I
Subtopic:  Cloning Vector |
 84%
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NEET - 2023
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Which of the following statement is incorrect above Agrobacterium tumefaciens ?
1. It is used to deliver gene of interest in both prokaryotic as well as eukaryotic host cells.
2. 'Ti' plasmid from Agrobacterium tumefaciens used for gene transfer is not pathogenic to plant cells.
3. It transforms normal plant cells into tumor cells
4. It delivers 'T-DNA' into plant cell
Subtopic:  Transforming Plant & Animal Cell |
 59%
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NEET - 2023
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 Match List - I with List - II
List-I List-II
(A) Kanamycin (I) Delivers genes into animal cells
(B) ClaI (II) Selectable marker
(C) Disarmed retroviruses (III) Restriction site
(D) Kanamycin Rgene (IV) Antibiotic resistance
Choose the correct answer from the options given below:
Options: (A) (B) (C) (D)
1. II III I IV
2. III I IV II
3. IV III I II
4. II IV I III

Subtopic:  Cloning Vector |
 52%
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NEET - 2023
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 Which of the following can act as molecular scissors?
1.Restriction enzymes2.DNA ligase
3.RNA polymerase4.DNA polymerase
Subtopic:  Restriction Enzymes - Main Enzymes |
 94%
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NEET - 2023
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Given below are two statements: one is labelled as Assertion (A) and the other is labelled as Reason (R) 
 
Assertion (A): Polymerase chain reaction is used in DNA amplification.
Reason (R): The ampicillin resistant gene is used as a selectable marker to check transformation. 

In the light of the above statements, choose the correct answer from the options given below: 
 
1. (A) is not correct but (R) is correct. 
2. Both (A) and (R) are correct and (R) is the correct explanation of (A). 
3. Both (A) and (R) are correct but (R) is not the correct explanation of (A).
4. (A) is correct but (R) is not correct.
Subtopic:  Selectable Markers: I | Polymerase Chain Reaction: PCR |
 75%
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NEET - 2022
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NEET 2023 - Target Batch - Aryan Raj Singh
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