(a) | Agarose is a natural polymer obtained from sea-weed. |
(b) | The separation of DNA molecules in agarose-gel electrophoresis depends on the size of DNA. |
(c) | The DNA migrates from negatively-charged electrode to the positively-charged electrode. |
(d) | The DNA migrates from positively-charged electrode to the negatively-charged electrode. |
1. | (a) and (b) only | 2. | (a), (b) and (c) only |
3. | (a), (b) and (d) only | 4. | (b), (c) and (d) only |
a. | They float freely in the cytoplasm of certain bacterial cells. |
b. | They have the ability to replicate, independent of the genomic DNA. |
c. | Herbert Boyer developed a method of removing and reinserting them in the cells. |
d. | They are circular ssDNA molecules. |
Column I | Column II | ||
a. | Salmonella typhimurium | (i) | Source of EcoRI |
b. | Bacillus thuringiensis | (ii) | Used in developing transgenic tobacco plant |
c. | Agrobacterium tumefaciens |
(iii) | Used in creating first recombinant DNA |
d. | Escherichia coli | (iv) | Gene used in creating biopesticide |
Column I | Column II | ||
a. | Restriction endonuclease | (i) | Hind II |
b. | First recombinant DNA | (ii) | Obtained from strain RY13 |
c. | First restriction endonuclease | (iii) | Isolated in 1963 |
d. | EcoRI | (iv) | Developed in 1972 |
Statement (A): | DNA cannot pass through cell membranes as it is a hydrophobic molecule. |
Statement (B): | Treatment with Ca2+ ions increases the efficiency with which DNA enters into the bacterium. |