Restriction endonucleases:
1. | Are used in genetic engineering for ligating two DNA molecules. |
2. | Are used for in vitro DNA synthesis. |
3. | Are synthesized by bacteria as part of their defense mechanism. |
4. | Are present in mammalian cells for the degradation of DNA when the cell dies. |
The Ti plasmid is often used for making transgenic plants. This plasmid is found in:
1. Rhizobium of the roots of leguminous plants
2. Agrobacterium
3. Yeast as a 2 µm plasmid
4. Azotobacter
Function of Nucleases:
1. | Break the polynucleotide chain by breaking each terminal nucleotide |
2. | Breaks phosphodiester bond |
3. | Breaks peptide bonds |
4. | Breaks ester bonds |
Hybridoma is:
1. | Collection of DNA from DNA |
2. | Collection of RNA from DNA |
3. | A fusion of tumour sex cell with non tumour sex cell |
4. | A fusion of tumour somatic cell with non tumour somatic cell |
The bacteria generally used for genetic engineering is:
1. Agrobacterium
2. Bacillus
3. Pseudomonas
4. Clostridium
A giant rat is formed in the laboratory, what is the reason:
1. Gene mutation
2. Gene synthesis
3. Gene manipulation
4. Gene replication
Plasmid has been used as a vector because:
1. | It is circular DNA that has the capacity to join to eukaryotic DNA |
2. | It can move between prokaryotic and eukaryotic cells |
3. | Both ends show replication |
4. | It has an antibiotic resistance gene |
In bacteria, the plasmid is:
1. Extrachromosomal material
2. Main DNA
3. Non-functional DNA
4. Repetitive gene
Which of the following enzymes are used to join bits of DNA:
1. Ligase
2. Primase
3. DNA polymerase
4. Endonuclease
Manipulation of DNA in genetic engineering became possible due to the discovery of:
1. Restriction endonuclease
2. DNA ligase
3. Transcriptase
4. Primase