What limitation of traditional hybridization procedures used in plant and animal breeding can be overcome by recombinant DNA procedures?
I: | Inclusion and multiplication of undesirable genes |
II: | Inability of inclusion of desirable traits from other species |
1. | Only I | 2. | Only II |
3. | Both I and II | 4. | Neither I nor II |
A piece of DNA, somehow transferred into an alien organism, will be able to multiply itself in the progeny cells of organism if:
1. | It includes the centromere |
2. | It becomes a part of chromosome |
3. | It does not contain any intron sequences |
4. | It is a single stranded polynucleotide |
The work of which of the following scientists led to the establishment of the discipline of modern biotechnology?
1. | Banting and Best | 2. | Bolivar and Rodriguez |
3. | Hershey and Chase | 4. | Cohen and Boyer |
What controls the copy number of linked DNA if plasmid is used as a vector in bacterial transformation?
1. | Ori | 2. | rop |
3. | Selectable markers | 4. | Promoter in the insert |
In the given diagram of pBR322, the location of the recognition sequences of BamH I and Sal I are represented by the numbers:
1. | 1 and 2 | 2. | 3 and 4 |
3. | 6 and 7 | 4. | 5 and 8 |
A reporter enzyme used in biotechnology includes all the following except:
1. Green Fluorescent Protein
2. Luciferase
3. Beta Galactosidase
4. Chloramphenicol acetyl transferase
Identify (a) and (b) bioreactors respectively in the given diagram:
1. Simple stirred-tank and sparged stirred – tank
2. Sparged stirred - tank and simple stirred-tank
3. Airlift and tower
4. Tower and airlift
Gene cloning occurs:
1. | when a phage transfers bacterial DNA from one bacterium to another. |
2. | when a bacterium takes up DNA from the surrounding fluid. |
3. | when a bacterium carrying a recombinant plasmid reproduces, thus allowing for the production of multiple copies of the recombinant plasmid. |
4. | when DNA is produced from an RNA template. |
If any protein-encoding gene is expressed in a heterologous host, the protein formed is called:
1. | Recombinant protein | 2. | Native protein |
3. | Pro-protein | 4. | Exotic protein |
In recombinant DNA experiments, a vector:
1. | carries DNA into a new cell |
2. | links together newly joined fragments of DN |
3. | makes millions of copies of a specific segment of DNA |
4. | separates fragments of DNA by their length and electrical charges |