For the multiplication of any alien piece of DNA in an organism, it needs to be part of a chromosome that has a specific:
1. | Telomeric sequence | 2. | Multiple cloning sites |
3. | Ori | 4. | Selectable marker |
The first type II restriction endonuclease whose functioning depended on a specific DNA nucleotide sequence was:
1. | EcoRI | 2. | HindII |
3. | SmaI | 4. | BamHI |
The following palindrome is recognized by the restriction enzyme:
1. | BamHI | 2. | EcoRI |
3. | HindII | 4. | PstI |
A method used only for transforming animal cells is:
1. | Biolistics | 2. | Microinjection |
3. | Use of virus | 4. | Agrobacterium mediation |
Lysozyme should be used when isolating DNA in a pure form from:
1. | A bacterial cell | 2. | A fungal cell |
3. | A plant cell | 4. | An animal cell |
In recombinant DNA experiments, a vector:
1. | carries DNA into a new cell |
2. | links together newly joined fragments of DN |
3. | makes millions of copies of a specific segment of DNA |
4. | separates fragments of DNA by their length and electrical charges |
If any protein-encoding gene is expressed in a heterologous host, the protein formed is called:
1. | Recombinant protein | 2. | Native protein |
3. | Pro-protein | 4. | Exotic protein |
Gene cloning occurs:
1. | when a phage transfers bacterial DNA from one bacterium to another. |
2. | when a bacterium takes up DNA from the surrounding fluid. |
3. | when a bacterium carrying a recombinant plasmid reproduces, thus allowing for the production of multiple copies of the recombinant plasmid. |
4. | when DNA is produced from an RNA template. |
Identify (a) and (b) bioreactors respectively in the given diagram:
1. Simple stirred-tank and sparged stirred – tank
2. Sparged stirred - tank and simple stirred-tank
3. Airlift and tower
4. Tower and airlift
A reporter enzyme used in biotechnology includes all the following except:
1. Green Fluorescent Protein
2. Luciferase
3. Beta Galactosidase
4. Chloramphenicol acetyl transferase