- 1(current)
Q. No.'Insertional inactivation' is used for
| 1. | Selection of recombinants |
| 2. | Producing pest resistant crops |
| 3. | Enhancing nutritional value of food |
| 4. | Reducing post harvest losses |
Subtopic: Selection of Recombinant Transformants |
89%
Level 1: 80%+
Hints
Selection of recombinants is based on all except one. Find out the exception
| 1. | Expression and non-expression of genes encoding for tetracycline-resistant compound |
| 2. | Expression and non-expression of genes encoding for insulin-resistant compound |
| 3. | Expression and non-expression of genes encoding for ampicillin-resistant compound |
| 4. | Insertional inactivation |
Subtopic: Selection of Recombinant Transformants |
81%
Level 1: 80%+
Hints
When recombinant DNA is inserted within the coding sequence of an enzyme β-galactosidase. This results in the following except
| 1. | Insertional inactivation |
| 2. | Recombinant colonies do not produce any color |
| 3. | Inactivation of enzyme |
| 4. | Chromogenic substrate is converted into product by recombinant colonies |
Subtopic: Selection of Recombinant Transformants |
65%
Level 2: 60%+
Hints
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If a foreign gene is inserted into pBR322 by using restriction enzyme BamHI, the selection process, for identifying recombinants requires plating on all except
| 1. | Ampicillin rich medium |
| 2. | Tetracycline rich medium |
| 3. | X-gal rich medium |
| 4. | Nutrient medium without antibiotics |
Subtopic: Selection of Recombinant Transformants |
Level 3: 35%-60%
Hints
The colonies of recombinant bacteria appear white in contrast to blue colonies of non-recombinant bacteria because of
| 1. | Non-recombinant bacteria containing -galactosidase |
| 2. | Insertional inactivation of -galactosidase in non-recombinant bacteria |
| 3. | Insertional inactivation of β-galactosidase in recombinant bacteria |
| 4. | Inactivation of glycosidase enzyme in recombinant bacteria |
Subtopic: Selectable Markers: III | Selection of Recombinant Transformants | Selectable Markers |
70%
Level 2: 60%+
NEET - 2013
Hints
- 1(current)
Q. No.
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