Q. No.The primary role of restriction enzymes in genetic engineering is:
1. DNA replication
2. DNA repair
3. DNA cutting at specific sites
4. DNA ligation
1. DNA replication
2. DNA repair
3. DNA cutting at specific sites
4. DNA ligation
Subtopic: Introduction & History: I | Introduction & History: II | Restriction Enzymes - Main Enzymes |
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Level 1: 80%+
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The common restriction enzyme EcoRI recognizes the palindromic sequence:
1. GAATTC
2. CCCGGG
3. AAGCTT
4. TGGCCA
1. GAATTC
2. CCCGGG
3. AAGCTT
4. TGGCCA
Subtopic: Restriction Enzymes - Main Enzymes |
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Why are restriction enzymes referred to as "molecular scissors"?
| 1. | Because they randomly degrade all DNA molecules inside a cell. |
| 2. | Because they attach new DNA segments to a target sequence during genetic recombination. |
| 3. | Because they cut DNA at specific recognition sequences, allowing precise genetic modifications. |
| 4. | Because they unwind and separate DNA strands during replication. |
Subtopic: Restriction Enzymes - Main Enzymes |
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The strain of Escherechia coli from which the restriction enzyme EcoR I has been isolated is:
1. DH5α
2. RY 13
3. NC101
4. K1
1. DH5α
2. RY 13
3. NC101
4. K1
Subtopic: Restriction Enzymes - Main Enzymes |
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Given below is a sample of a portion of DNA strand. What is so special shown in it?
5' GAATTC 3'
3' CTTAAG 5'
1. Replication completed
2. Deletion mutation
3. Start codon at the 5' end
4. Palindromic sequence of base pairs
Subtopic: Restriction Enzymes - Main Enzymes |
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Each restriction enzyme cleaves a molecule only at
1. methyl groups.
2. the ends of genes.
3. a particular nucleotide sequence.
4. the time of DNA replication.
1. methyl groups.
2. the ends of genes.
3. a particular nucleotide sequence.
4. the time of DNA replication.
Subtopic: Restriction Enzymes - Main Enzymes |
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A specific recognition sequence identified by endonucleases to make cuts at specific positions within the DNA is:
| 1. | Palindromic Nucleotide sequences |
| 2. | Poly(A) tail sequences |
| 3. | Degenerate primer sequence |
| 4. | Okazaki sequences |
Subtopic: Restriction Enzymes - Main Enzymes |
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The restriction endonuclease enzymes have been isolated from:
| 1. | Bacteria | 2. | Yeasts |
| 3. | Algae | 4. | Fungi |
Subtopic: Restriction Enzymes - Main Enzymes |
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There is a restriction endonuclease called EcoRI. What does ‘co’ part in it stand for?
| 1. | Colon | 2. | Coelom |
| 3. | Coenzyme | 4. | coli |
Subtopic: Restriction Enzymes - Main Enzymes |
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Level 1: 80%+
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Bacterial cells add methyl groups to their DNA in order to:
1. clone their DNA
2. activate promoters
3. form polycisronic mRNAs
4. protect its DNA from own restriction enzymes
1. clone their DNA
2. activate promoters
3. form polycisronic mRNAs
4. protect its DNA from own restriction enzymes
Subtopic: Restriction Enzymes - Main Enzymes |
88%
Level 1: 80%+
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