For transformation, micro particles coated with DNA to be bombarded with gene gun are made up of-
1. Silver or Platinum
2. Gold or Tungsten
3. Silicon and Platinum
4. Platinum or Zinc
Subtopic:  Transforming Plant & Animal Cell |
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Level 1: 80%+
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Two microbes found to be very useful in gene engineering are 
1. Escherichia coli and Agrobacterium tumefaciens.
2. Vibrio cholerae and a tailed bacteriophage.
3. Diplococcus sp. And Pseudomonas sp.
4. Crown gall bacterium and Caenorphaditiselegans. 

Subtopic:  Transforming Plant & Animal Cell |
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Level 1: 80%+
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Plant cells are bombarded with high-velocity microparticles of X coated with Y in a method called Z. Choose the option that fills the blanks correctly.

X Y Z
1. Gold or tungsten  DNA Biolistics
2. Platinum and gold RNA Biolistics
3. Gold or silver RNA Gene gun
4. Platinum or silver  RNA Gene gun
Subtopic:  Transforming Plant & Animal Cell |
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The introduction of tDNA into plants involves:

1. infection of the plant by Agrobacterium tumefaciens
2. Altering the pH of the soil, then heat-shocking the plants
3. Exposing the plants to cold for a brief period
4. Allowing the plant roots to stand in water
Subtopic:  Transforming Plant & Animal Cell |
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Level 1: 80%+
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Ti plasmid is found in:
1. Escherichia coli
2. Agrobacterium tumefaciens
3. Salmonella typhimurium
4. Haemophilus influenzae
Subtopic:  Transforming Plant & Animal Cell |
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Level 1: 80%+
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To make the host cells competent, they are treated with specified concentration of:
1. Ca+2 2. Na+
3. K+ 4. Cl-
Subtopic:  Transforming Plant & Animal Cell |
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Level 1: 80%+
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Which of the following techniques is commonly used for the production of genetically modified dicot plants? 
1. Polymerase Chain Reaction (PCR) 
2. Southern blotting 
3. Agarose gel electrophoresis 
4. Agrobacterium-mediated gene transfer
Subtopic:  Transforming Plant & Animal Cell |
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Level 1: 80%+
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Complete the analogy with respect to the method of gene transfer and the most suitable host cell for it
Microinjection : Animal cell :: Biolistics:______
Select the correct option:
1. Bacteria
2. Plant cell
3. Bacteriophage
4. Fungal cell
Subtopic:  Transforming Plant & Animal Cell |
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Level 1: 80%+
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Method in which recombinant DNA is directly injected into the nucleus of animal cell by using microneedles is called
1. Gene Gun method 
2. Biolistic method
3. Microinjection method
4. Electroporation 
Subtopic:  Transforming Plant & Animal Cell | Cloning Vector |
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Match List-I with List-II
List-I List-II
(a) Gene gun (i) Replacement of a faulty gene by a normal healthy gene
(b)  Gene therapy (ii) Used for transfer of gene
(c) Gene cloning (iii) Total DNA in the cells of an organism
(d) Genome (iv) To obtain identical copies of a particular DNA molecule

Choose the correct answer from the options given below:
(a) (b) (c) (d)
1. (ii) (i) (iv) (iii)
2. (i) (iii) (ii) (iv)
3. (iv) (i) (iii) (ii)
4. (ii) (iii) (iv) (i)
Subtopic:  Transforming Plant & Animal Cell |
 92%
Level 1: 80%+
NEET - 2022
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